Rat dentin matrix protein 3 is a compound protein of rat dentin sialoprotein and phosphophoryn

Anne George*, Radhika Srinivasan, Seetha R. Thotakura, Kai Liu, Arthur Veis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

During cloning of the rat incisor phosphophoryn gene, several clones were identified with a PP antibody. One of the clones (2PP) was shown to encode a PP denoted as dentin matrix protein 2, DMP2. We have now sequenced another clone, which appears to be bifunctional, or at minimum, has two distinct domains. The 5' region encodes for dentin sialoprotein (DSP), while the 3' region encodes a small or 'mini' PP. There is no stop codon between these domains. The whole gene has been named Dmp3, in keeping with the current nomenclature adopted in our laboratory. The previously reported Dmp2 gene does not encode a DSP domain but has a 114 amino acid carboxy terminal sequence identical except for a single residue with that of the DMP3. Thus, Dmp2, Dmp3 and their corresponding proteins, probably represent related members of a multigene family. There is no evidence for differential splicing. Since the DSP isolated from dentin does not carry the mini-PP domain, it must be cleaved postranslationally from DMP3.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalConnective tissue research
Volume40
Issue number1
DOIs
StatePublished - 1999

Keywords

  • Biomineralization
  • DMP2
  • Dentin phosphoprotein
  • Dentin sialoprotein
  • Phosphophoryn

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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