Rat intestinal epithelial cells present major histocompatibility complex allopeptides to primed T cells

Judson M. Brandeis; Mohamed H. Sayegh; Lorenzo Gallon; Richard S. Blumberg; Charles B. Carpenter

doi:10.1016/0016-5085(94)90560-6

Rat intestinal epithelial cells present major histocompatibility complex allopeptides to primed T cells

Judson M. Brandeis, Mohamed H. Sayegh, Lorenzo Gallon, Richard S. Blumberg, Charles B. Carpenter^*

^*Corresponding author for this work

Medicine, Nephrology Division

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Background/Aims: Intestinal epithelial cells present protein antigens to primed T cells in vitro. The aim of this study was to investigate whether intestinal epithelial cells present peptide antigens in vitro and in vivo after oral administration. Methods: Small intestinal epithelial cells from naive LEW (RT1¹) rats pulsed in vitro with a synthetic immunogenic major histocompatibility complex allopeptide, RT1.D^uβ20-44, or in vivo by oral administration of the peptide were tested for their ability to induce specific proliferation of LEW T cells primed in vivo to RT1.D^uβ20-44. Results: In vitro pulsed intestinal epithelial cells induced specific proliferation of RT1.D^uβ20-44-primed T cells. Intestinal epithelial cells isolated from LEW rats that received a single oral dose of RT1.D^uβ20-44 18 hours earlier also induced specific proliferation of RT1.D^uβ20-44-primed LEW T cells. Furthermore, epithelial cells harvested from LEW rats that received WF (RT1^u) splenocytes orally 18 hours earlier induced specific proliferation of RT1.D^uβ20-44-primed LEW T cells. Conclusions: Intestinal epithelial cells take up processed alloantigen in vitro and in vivo for presentation as peptides to primed T cells. These observations provide a novel approach to study the role of the intestinal immune system in immune regulation in vivo.

Original language	English (US)
Pages (from-to)	1537-1542
Number of pages	6
Journal	Gastroenterology
Volume	107
Issue number	5
DOIs	https://doi.org/10.1016/0016-5085(94)90560-6
State	Published - Nov 1994

Funding

ASJC Scopus subject areas

Gastroenterology
Hepatology

Access to Document

10.1016/0016-5085(94)90560-6

Cite this

@article{58c6945e74634ae496ba57ebaed9445e,

title = "Rat intestinal epithelial cells present major histocompatibility complex allopeptides to primed T cells",

abstract = "Background/Aims: Intestinal epithelial cells present protein antigens to primed T cells in vitro. The aim of this study was to investigate whether intestinal epithelial cells present peptide antigens in vitro and in vivo after oral administration. Methods: Small intestinal epithelial cells from naive LEW (RT11) rats pulsed in vitro with a synthetic immunogenic major histocompatibility complex allopeptide, RT1.Duβ20-44, or in vivo by oral administration of the peptide were tested for their ability to induce specific proliferation of LEW T cells primed in vivo to RT1.Duβ20-44. Results: In vitro pulsed intestinal epithelial cells induced specific proliferation of RT1.Duβ20-44-primed T cells. Intestinal epithelial cells isolated from LEW rats that received a single oral dose of RT1.Duβ20-44 18 hours earlier also induced specific proliferation of RT1.Duβ20-44-primed LEW T cells. Furthermore, epithelial cells harvested from LEW rats that received WF (RT1u) splenocytes orally 18 hours earlier induced specific proliferation of RT1.Duβ20-44-primed LEW T cells. Conclusions: Intestinal epithelial cells take up processed alloantigen in vitro and in vivo for presentation as peptides to primed T cells. These observations provide a novel approach to study the role of the intestinal immune system in immune regulation in vivo.",

author = "Brandeis, {Judson M.} and Sayegh, {Mohamed H.} and Lorenzo Gallon and Blumberg, {Richard S.} and Carpenter, {Charles B.}",

year = "1994",

month = nov,

doi = "10.1016/0016-5085(94)90560-6",

language = "English (US)",

volume = "107",

pages = "1537--1542",

journal = "Gastroenterology",

issn = "0016-5085",

publisher = "W.B. Saunders Ltd",

number = "5",

}

TY - JOUR

T1 - Rat intestinal epithelial cells present major histocompatibility complex allopeptides to primed T cells

AU - Brandeis, Judson M.

AU - Sayegh, Mohamed H.

AU - Gallon, Lorenzo

AU - Blumberg, Richard S.

AU - Carpenter, Charles B.

PY - 1994/11

Y1 - 1994/11

N2 - Background/Aims: Intestinal epithelial cells present protein antigens to primed T cells in vitro. The aim of this study was to investigate whether intestinal epithelial cells present peptide antigens in vitro and in vivo after oral administration. Methods: Small intestinal epithelial cells from naive LEW (RT11) rats pulsed in vitro with a synthetic immunogenic major histocompatibility complex allopeptide, RT1.Duβ20-44, or in vivo by oral administration of the peptide were tested for their ability to induce specific proliferation of LEW T cells primed in vivo to RT1.Duβ20-44. Results: In vitro pulsed intestinal epithelial cells induced specific proliferation of RT1.Duβ20-44-primed T cells. Intestinal epithelial cells isolated from LEW rats that received a single oral dose of RT1.Duβ20-44 18 hours earlier also induced specific proliferation of RT1.Duβ20-44-primed LEW T cells. Furthermore, epithelial cells harvested from LEW rats that received WF (RT1u) splenocytes orally 18 hours earlier induced specific proliferation of RT1.Duβ20-44-primed LEW T cells. Conclusions: Intestinal epithelial cells take up processed alloantigen in vitro and in vivo for presentation as peptides to primed T cells. These observations provide a novel approach to study the role of the intestinal immune system in immune regulation in vivo.

AB - Background/Aims: Intestinal epithelial cells present protein antigens to primed T cells in vitro. The aim of this study was to investigate whether intestinal epithelial cells present peptide antigens in vitro and in vivo after oral administration. Methods: Small intestinal epithelial cells from naive LEW (RT11) rats pulsed in vitro with a synthetic immunogenic major histocompatibility complex allopeptide, RT1.Duβ20-44, or in vivo by oral administration of the peptide were tested for their ability to induce specific proliferation of LEW T cells primed in vivo to RT1.Duβ20-44. Results: In vitro pulsed intestinal epithelial cells induced specific proliferation of RT1.Duβ20-44-primed T cells. Intestinal epithelial cells isolated from LEW rats that received a single oral dose of RT1.Duβ20-44 18 hours earlier also induced specific proliferation of RT1.Duβ20-44-primed LEW T cells. Furthermore, epithelial cells harvested from LEW rats that received WF (RT1u) splenocytes orally 18 hours earlier induced specific proliferation of RT1.Duβ20-44-primed LEW T cells. Conclusions: Intestinal epithelial cells take up processed alloantigen in vitro and in vivo for presentation as peptides to primed T cells. These observations provide a novel approach to study the role of the intestinal immune system in immune regulation in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0028143458&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028143458&partnerID=8YFLogxK

U2 - 10.1016/0016-5085(94)90560-6

DO - 10.1016/0016-5085(94)90560-6

M3 - Article

C2 - 7926518

AN - SCOPUS:0028143458

SN - 0016-5085

VL - 107

SP - 1537

EP - 1542

JO - Gastroenterology

JF - Gastroenterology

IS - 5

ER -

Rat intestinal epithelial cells present major histocompatibility complex allopeptides to primed T cells

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this