Rat intestinal epithelial cells present major histocompatibility complex allopeptides to primed T cells

Judson M. Brandeis, Mohamed H. Sayegh, Lorenzo Gallon, Richard S. Blumberg, Charles B. Carpenter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Background/Aims: Intestinal epithelial cells present protein antigens to primed T cells in vitro. The aim of this study was to investigate whether intestinal epithelial cells present peptide antigens in vitro and in vivo after oral administration. Methods: Small intestinal epithelial cells from naive LEW (RT11) rats pulsed in vitro with a synthetic immunogenic major histocompatibility complex allopeptide, RT1.Duβ20-44, or in vivo by oral administration of the peptide were tested for their ability to induce specific proliferation of LEW T cells primed in vivo to RT1.Duβ20-44. Results: In vitro pulsed intestinal epithelial cells induced specific proliferation of RT1.Duβ20-44-primed T cells. Intestinal epithelial cells isolated from LEW rats that received a single oral dose of RT1.Duβ20-44 18 hours earlier also induced specific proliferation of RT1.Duβ20-44-primed LEW T cells. Furthermore, epithelial cells harvested from LEW rats that received WF (RT1u) splenocytes orally 18 hours earlier induced specific proliferation of RT1.Duβ20-44-primed LEW T cells. Conclusions: Intestinal epithelial cells take up processed alloantigen in vitro and in vivo for presentation as peptides to primed T cells. These observations provide a novel approach to study the role of the intestinal immune system in immune regulation in vivo.

Original languageEnglish (US)
Pages (from-to)1537-1542
Number of pages6
JournalGastroenterology
Volume107
Issue number5
DOIs
StatePublished - Nov 1994

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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