Rat urate oxidase: cloning and structural analysis of the gene and 5'-flanking region

Xuedong Wang, Hiroko Kawano, Keith Alvares, P. Gopal Reddy, Helen Getto, M. Sambasiva Rao, Janardan K. Reddy*

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The structural gene (UOX) encoding rat urate oxidase (UOX) spans at least 23 kb and is composed of eight exons and seven introns. All of the exon-intron splice junction sequences conformed to the GT/AG consensus established for eukaryotic genes. The transcription start point (tsp) was determined using S1-type nuclease protection riboprobe, and assigned to an adenine 54 nucleotides (nt) upstream of the ATG start codon. A 456-bp 5' -terminal fragment, starting at the ATG codon, carries a putative TATA (ATAAAA) sequence at -32, and two putative 'CAAT box' sequences at -62 and -71 bp upstream from the tsp. No sequence resembling 'GC' box hexanucleotides (GGGCGG or CCGCCC) was found. The structural features of the 5' -flanking region of the UOX gene are distinct from the 5' -flanking sequences of peroxisomal β-oxidation system genes which contain one or more 'GC' box elements but lack TATA- and CAAT-like features [Osumi et al., J. Biol. Chem. 262 (1987) 8138-8143; Ishii et al., J. Biol. Chem. 262 (1987) 8144-8150]. The 5' -flanking region of the UOX gene reveals a sequence, TTAGTAATT at nt -276 from the tsp, which appears to be complementary to the underlined part of the liver-specific LF-B1/HNF-1 consensus sequence, GTTAATNATTAAC (where N = A, C, T, G or no nt).

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalGene
Volume97
Issue number2
DOIs
StatePublished - Jan 15 1991

Keywords

  • Recombinant DNA
  • S1-type mapping
  • exon
  • intron
  • peroxisome
  • splice junction
  • tissue specificity

ASJC Scopus subject areas

  • Genetics

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