Re-expression of elafin in 21MT2 breast carcinomas by phorbol 12- myristate 13-acetate is mediated by the Ap1 site in the elafin promoter

Ming Zhang, David Magit, Arthur B. Pardee, Ruth Sager

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

elafin is a unique elastase inhibitor. It is differentially expressed at the transcriptional level in human normal mammary epithelial cells and carcinomas. The elafin gene is induced by PMA in 21MT2 breast tumor cells. By deletion analysis and mutagenesis, we have identified the Ap1 site in the promoter as the cis element mediating transcriptional activation of elafin in 70N normal breast cells and its induction by phorbol 12-myristate 13-acetate (PMA) in 21MT2 breast tumors. PMA treatment induces AP1 factor expression, which binds to the Ap1 site of the elafin promoter. Mutation of this Ap1 abolishes the capability of induction by PMA. Furthermore, our data provide a basis for therapeutic manipulation of proteinase inhibitors such as elafin.

Original languageEnglish (US)
Pages (from-to)4631-4636
Number of pages6
JournalCancer Research
Volume57
Issue number20
StatePublished - Oct 15 1997

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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