Reactive oxygen and nitrogen species: Weapons of neuronal destruction

Joe Mazzulli, Summer Lind, Harry Ischiropoulos

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Recent advancements in understanding the biochemical actions of reactive oxygen and nitrogen species (RONS) have identified many biological targets that reliably serve as indices of oxidative and nitrative stress. Oxidative stress occurs when uncontrolled and excessive amounts of oxidants are produced, resulting in the formation of oxidized macromolecules, whereas nitrative stress occurs when excessive reactive nitrogen species are produced resulting in the formation of nitrated macromolecules. The most frequently detected oxidant-modified biological targets include: 1) the polyunsaturated fatty acid oxidation products, isoprostanes and the electrophiles 4-hydroxy-2(E)nonenal (4-HNE) and malondialdehyde (MDA), 2) 8-hydroxy-2′-deoxyguanosine, the most studied product of DNA oxidation, and 3) oxidatively modified proteins including advanced glycation endproducts (AGEs) and proteins with reactive carbonyl side groups. Modification by reactive nitrogen species (reactive species derived from nitric oxide (NO) or metabolites of NO such as dinitrogen trioxide, nitrous acid, nitrogen dioxide, and peroxynitrite) lead to the formation of nitrated macromolecules by the addition of a nitro(-NO2) group. Biological targets modified by nitration include: 1) tyrosine and tryptophan residues in proteins, 2) polyunsaturated fatty acids, 3) DNA bases, and 4) sugars. All of these oxidative modifications can be readily detected in biological samples, providing molecular footprints to track the generation and actions of RONS. Moreover, the identification and quantification of these biomarkers may provide a reliable assessment of disease risk and severity.

Original languageEnglish (US)
Title of host publicationOxidative Stress and Age-Related Neurodegeneration
EditorsY Luo, L Packer
PublisherCRC Press
Number of pages14
ISBN (Electronic)9781420026559
ISBN (Print)9780849337253
StatePublished - Jan 1 2005

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)


Dive into the research topics of 'Reactive oxygen and nitrogen species: Weapons of neuronal destruction'. Together they form a unique fingerprint.

Cite this