Real-time analysis of clathrin-mediated endocytosis during cell migration

Joshua Z. Rappoport, Sanford M. Simon*

*Corresponding author for this work

Research output: Contribution to journalReview article

134 Scopus citations

Abstract

Simultaneous dual-color total-internal-reflection fluorescence microscopy (TIR-FM) was performed to analyze the internalization and distribution of markers for clathrin-mediated endocytosis (clathrin, dynamin1, dynamin2 and transferrin) in migrating cells. In MDCK cells, which endogenously express dynamin2, the dynamin2-EGFP fluorescence demonstrated identical spatial and temporal behavior as clathrin both prior to and during internalization. By contrast, in the same cells, the neuronal dynamin1 only localized with clathrin just prior to endocytosis. In migrating cells, each endocytic marker was polarized towards the leading edge, away from the lagging edge. These observations suggest a re-evaluation of the functional differences between dynamin1 and dynamin2, and of the role of clathrin-mediated endocytosis in cell migration.

Original languageEnglish (US)
Pages (from-to)847-855
Number of pages9
JournalJournal of cell science
Volume116
Issue number5
DOIs
StatePublished - Mar 1 2003

Keywords

  • Cell migration
  • Clathrin
  • Dynamin
  • Endocytosis
  • Evanescent-wave microscopy
  • Total internal reflection fluorescence microscopy (TIR-FM)

ASJC Scopus subject areas

  • Cell Biology

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