Abstract
Mechanical forces such as fluid shear have been shown to enhance cell growth and differentiation, but knowledge of their mechanistic effect on cells is limited because the local flow patterns and associated metrics are not precisely known. Here we present real-time, non-invasive measures of local hydrodynamics in 3D biomaterials based on nuclear magnetic resonance. Microflow maps were further used to derive pressure, shear and fluid permeability fields. Finally, remodeling of collagen gels in response to precise fluid flow parameters was correlated with structural changes. It is anticipated that accurate flow maps within 3D matrices will be a critical step towards understanding cell behavior in response to controlled flow dynamics.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1980-1986 |
| Number of pages | 7 |
| Journal | Biomaterials |
| Volume | 34 |
| Issue number | 8 |
| DOIs | |
| State | Published - Mar 2013 |
Funding
This study was supported by funds from the UCLA Stem Cell Center Innovator Award to M.L.I.A. and the Ruth L. Kirschstein National Research Service Awar d ( T32HL69766 to J.J.M. and O.D.V.N.). Research funding to L.S.B. from the Camille & Henry Dreyfus Foundation and the Arnold and Mabel Beckman Foundation are acknowledged. The authors have no conflicting financial interests to disclose. L.S.B., J.M., M.L.I.A. designed experiments; L.S.B., J.M., O.N., M.L., performed experiments; L.S.B., A.W., O.N., J.M. designed the flow chambers; L.S.B., J.M. conducted data processing and error analysis; J.M., L.S.B. wrote and tested flow pulse sequence; K.Y. performed computations on the fluid flow field; L.S.B., J.M., M.L.I.A. wrote the paper. The authors declare no competing financial interests. We thank Melody Swartz and Petros Koumoutsakos for critical reviews on the manuscript.
Keywords
- 3D scaffold
- Flow
- Fluid permeability
- Hydrogel
- NMR
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Ceramics and Composites
- Biomaterials
- Mechanics of Materials