Real-time qPCR for chimerism assessment in allogeneic hematopoietic stem cell transplants from unrelated adult and double umbilical cord blood

Olga Frankfurt, Jennifer R. Zitzner, Anat R. Tambur*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Allogeneic hematopoietic stem cell transplantation is the standard therapy for patients with various malignant hematologic disorders. A successful treatment results in complete engraftment of donor cells in the absence of the patient's own hematopoietic system. Chimerism analysis, aimed at determining the coexistence of genetically different cell populations, is considered a useful method to measure treatment success. A new, qPCR based, commercially available chimerism assay was recently introduced. Here we report our results of comparing STR with qPCR-based chimerism analysis, and assessment of sensitivity and reproducibility of the qPCR chimerism assay. A specific emphasis is put on analyzing chimerism in recipients of double cord blood transplantation. We conclude that the qPCR chimerism assay for engraftment monitoring is a reliable and sensitive assay. Advantages and limitations of the assay in its current format are summarized.

Original languageEnglish (US)
Pages (from-to)155-160
Number of pages6
JournalHuman Immunology
Volume76
Issue number2-3
DOIs
StatePublished - Mar 1 2015

Keywords

  • Chimerism
  • Engraftment-monitoring
  • QPCR
  • STR
  • Stem-cell-transplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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