TY - JOUR
T1 - Reanalysis of Antisera Specificities and Binding Characteristics of Rat Pituitary Hormone Assays
AU - Cheresman, Kerry L.
AU - Chatterton, Robert T.
PY - 1984/3/1
Y1 - 1984/3/1
N2 - Rat pituitary hormone radioimmunoassays (RIAs) are widely used in reproductive research, yet data on specificity and binding characteristics of many of the antisera are not widely available. This report characterizes one set of rat antisera supplied by the National Institutes of Health (USA). Rat follicle-stimulating hormone (FSH) and thyrotropin-stimulating hormone (TSH) antisera appear specific, but TSH exhibited significant competition in the rat luteinizing hormone (LH) assay. In addition, statistically significant nonparallelism was demonstrable in all three assay systems. This creates further problems in characterizing antisera cross-reactivity and may make potency estimates for pituitary standards inaccurate. (KEY WORDS: Rat, Pituitary, Radioimmunoassay).
AB - Rat pituitary hormone radioimmunoassays (RIAs) are widely used in reproductive research, yet data on specificity and binding characteristics of many of the antisera are not widely available. This report characterizes one set of rat antisera supplied by the National Institutes of Health (USA). Rat follicle-stimulating hormone (FSH) and thyrotropin-stimulating hormone (TSH) antisera appear specific, but TSH exhibited significant competition in the rat luteinizing hormone (LH) assay. In addition, statistically significant nonparallelism was demonstrable in all three assay systems. This creates further problems in characterizing antisera cross-reactivity and may make potency estimates for pituitary standards inaccurate. (KEY WORDS: Rat, Pituitary, Radioimmunoassay).
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U2 - 10.1080/01971528408062998
DO - 10.1080/01971528408062998
M3 - Article
C2 - 6425364
AN - SCOPUS:0021243406
SN - 1532-1819
VL - 5
SP - 59
EP - 70
JO - Journal of Immunoassay and Immunochemistry
JF - Journal of Immunoassay and Immunochemistry
IS - 1-2
ER -