Reanalysis of Antisera Specificities and Binding Characteristics of Rat Pituitary Hormone Assays

Kerry L. Cheresman, Robert T. Chatterton

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Abstract

Rat pituitary hormone radioimmunoassays (RIAs) are widely used in reproductive research, yet data on specificity and binding characteristics of many of the antisera are not widely available. This report characterizes one set of rat antisera supplied by the National Institutes of Health (USA). Rat follicle-stimulating hormone (FSH) and thyrotropin-stimulating hormone (TSH) antisera appear specific, but TSH exhibited significant competition in the rat luteinizing hormone (LH) assay. In addition, statistically significant nonparallelism was demonstrable in all three assay systems. This creates further problems in characterizing antisera cross-reactivity and may make potency estimates for pituitary standards inaccurate. (KEY WORDS: Rat, Pituitary, Radioimmunoassay).

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalJournal of Immunoassay
Volume5
Issue number1-2
DOIs
Publication statusPublished - Mar 1 1984

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ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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