Purpose Hyaluronic acid (HA) concentration decreases in human trabecular meshwork (TM) of primary open angle glaucoma (POAG) eyes as determined by previous biochemical and morphological studies. One possible mechanism for alteration in HA content may relate to RHAMM, which is required for cell motility in ras-transformed cells, fibroblasts and PC 12 neurons. Methods POAG and agematched control eyes were preserved in ethanol and the TM, ciliary body (CB) and iris were microdissected. To determine the presence of the RHAMM protein, Western blotting was carried out with a monoclonal anti-RHAMM antibody (3T3NMAb). Jksillu In the TM of normal eyes, the overall anti-RHAMM immunoreactivity decreased with age, whereas in the TM of POAG eyes a further decrease of RHAMM was observed. Similarly, the presence of RHAMM in the CB was reduced in two POAG eyes and completely absent in three eyes. A slight decrease of RHAMM was observed in the aging CB. Conclusions The observation of a decrease in the expression of the HA receptor, RHAMM, in POAG eyes may relate to the previously noted decrease in HA concentration in the TM. The decreased RHAMM expression may serve as a potential marker for or perhaps a possible contributor to the POAG disease process.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Dec 1 1997|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience