Recruitment of neutrophils during ige-dependent cutaneous late phase reactions in the mouse is mast cell-dependent partial inhibition of the reaction with antiserum against tumor necrosis factor-alpha

Barry K. Wershil*, Zhen Sheng Wang, John R. Gordon, Stephen J. Galli

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

282 Scopus citations

Abstract

Much of the clinically important pathology associated with IgE-dependent disorders is thought to reflect the actions of the blood-borne leukocytes recruited during these responses. To evaluate the extent to which mast cells are responsible for the leukocyte infiltration associated with IgE-dependent cutaneous reactions, we attempted to elicit these responses in normal mice, genetically mast cell-deficient W/Wv mice, and in W/Wv mice selectively repaired of their mast cell deficiency by the intradermal injection of cultured mast cells derived from the congenic normal (+/+) mice. We found that the tissue swelling associated with IgE-dependent passive cutaneous anaphylaxis reactions developed rapidly and diminished markedly from 2 to 4 h after antigen challenge, but remained detectable for at least 24 h after elicitation of the responses. Infiltration of leukocytes (predominantly neutrophils) also occurred at these sites, but reached maximal levels 6-12 h after antigen challenge, persisted at high levels for 24 h, and largely waned by 48 h. Virtually all of the tissue swelling and leukocyte infiltration associated with IgE-dependent cutaneous reactions was mast cell dependent. Intradermal injection of 40 U of recombinant murine TNF-α (rmTNF-α) elicited neutrophil infiltration similar in magnitude and kinetics to that observed after IgE-dependent mast cell degranulation. A rabbit anti-rmTNF-α (R anti-rmTNF-α) antiserum, which was able to inhibit 84% of the neutrophil infiltration observed after i.d. injection of rmTNF-α, inhibited IgE-, and mast cell-dependent leukocyte infiltration by 47±7% in three separate experiments. These findings indicate that TNF-α contributes to mast cell-dependent recruitment of leukocytes during IgE-dependent cutaneous late phase reactions, but suggest that other mast cell-associated mediators probably also contribute to this response.

Original languageEnglish (US)
Pages (from-to)446-453
Number of pages8
JournalJournal of Clinical Investigation
Volume87
Issue number2
DOIs
StatePublished - 1991

Keywords

  • Anaphylactic degranulation
  • Inflammation
  • Local mast cell reconstitution
  • Mast cell-deficient mice
  • Passive cutaneous anaphylaxis

ASJC Scopus subject areas

  • Medicine(all)

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