Reference maps of human es and ips cell variation enable high-throughput characterization of pluripotent cell lines

Christoph Bock, Evangelos Kiskinis, Griet Verstappen, Hongcang Gu, Gabriella Boulting, Zachary D. Smith, Michael Ziller, Gist F. Croft, MacKenzie W. Amoroso, Derek H. Oakley, Andreas Gnirke, Kevin Eggan*, Alexander Meissner

*Corresponding author for this work

Research output: Contribution to journalArticle

651 Scopus citations

Abstract

The developmental potential of human pluripotent stem cells suggests that they can produce disease-relevant cell types for biomedical research. However, substantial variation has been reported among pluripotent cell lines, which could affect their utility and clinical safety. Such cell-line-specific differences must be better understood before one can confidently use embryonic stem (ES) or induced pluripotent stem (iPS) cells in translational research. Toward this goal we have established genome-wide reference maps of DNA methylation and gene expression for 20 previously derived human ES lines and 12 human iPS cell lines, and we have measured the in vitro differentiation propensity of these cell lines. This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines. The combination of assays yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.

Original languageEnglish (US)
Pages (from-to)439-452
Number of pages14
JournalCell
Volume144
Issue number3
DOIs
StatePublished - Feb 4 2011

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Bock, C., Kiskinis, E., Verstappen, G., Gu, H., Boulting, G., Smith, Z. D., Ziller, M., Croft, G. F., Amoroso, M. W., Oakley, D. H., Gnirke, A., Eggan, K., & Meissner, A. (2011). Reference maps of human es and ips cell variation enable high-throughput characterization of pluripotent cell lines. Cell, 144(3), 439-452. https://doi.org/10.1016/j.cell.2010.12.032