Region-specific regulation of preproenkephalin mRNA in cultured astrocytes

David K. Batter*, John A. Kessler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Regulation of preproenkephalin (PPE) mRNA was examined in astrocytes cultured from several regions of the neonatal rat brain. Astrocytes from these regions expressed differing levels of PPE mRNA, with higher levels in astrocytes from the hypothalamus followed by frontal cortex and striatum. Further, PPE mRNA was regulated differently in hypothalamic than in striatal glia. Treatment of striatal astrocytes with the β-adrenergic agonist, isoproterenol, or with agents which directly increased intracellular cAMP (forskolin or 8-bromo-cAMP) elevated levels of PPE mRNA. By contrast, none of these treatments altered levels of PPE mRNA in hypothalamic astrocytes despite increasing cAMP levels 60-fold. These observations indicate that there is striking regional heterogeneity in the expression and regulation of PPE mRNA by astrocytes, suggesting that proenkephalin or its derived peptides help to mediate region-specific brain functions.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalMolecular Brain Research
Issue number1
StatePublished - Aug 1991


  • Astrocyte culture
  • Cyclic AMP
  • Glial heterogeneity
  • Opiate peptide precursor
  • Preproenkephalin mRNA
  • mRNA regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience


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