Region-specific regulation of preproenkephalin mRNA in cultured astrocytes

David K. Batter; John A. Kessler

doi:10.1016/0169-328X(91)90022-P

Region-specific regulation of preproenkephalin mRNA in cultured astrocytes

David K. Batter^*, John A. Kessler

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Regulation of preproenkephalin (PPE) mRNA was examined in astrocytes cultured from several regions of the neonatal rat brain. Astrocytes from these regions expressed differing levels of PPE mRNA, with higher levels in astrocytes from the hypothalamus followed by frontal cortex and striatum. Further, PPE mRNA was regulated differently in hypothalamic than in striatal glia. Treatment of striatal astrocytes with the β-adrenergic agonist, isoproterenol, or with agents which directly increased intracellular cAMP (forskolin or 8-bromo-cAMP) elevated levels of PPE mRNA. By contrast, none of these treatments altered levels of PPE mRNA in hypothalamic astrocytes despite increasing cAMP levels 60-fold. These observations indicate that there is striking regional heterogeneity in the expression and regulation of PPE mRNA by astrocytes, suggesting that proenkephalin or its derived peptides help to mediate region-specific brain functions.

Original language	English (US)
Pages (from-to)	65-69
Number of pages	5
Journal	Molecular Brain Research
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(91)90022-P
State	Published - Aug 1991

Keywords

Astrocyte culture
Cyclic AMP
Glial heterogeneity
Opiate peptide precursor
Preproenkephalin mRNA
mRNA regulation

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/0169-328X(91)90022-P

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title = "Region-specific regulation of preproenkephalin mRNA in cultured astrocytes",

abstract = "Regulation of preproenkephalin (PPE) mRNA was examined in astrocytes cultured from several regions of the neonatal rat brain. Astrocytes from these regions expressed differing levels of PPE mRNA, with higher levels in astrocytes from the hypothalamus followed by frontal cortex and striatum. Further, PPE mRNA was regulated differently in hypothalamic than in striatal glia. Treatment of striatal astrocytes with the β-adrenergic agonist, isoproterenol, or with agents which directly increased intracellular cAMP (forskolin or 8-bromo-cAMP) elevated levels of PPE mRNA. By contrast, none of these treatments altered levels of PPE mRNA in hypothalamic astrocytes despite increasing cAMP levels 60-fold. These observations indicate that there is striking regional heterogeneity in the expression and regulation of PPE mRNA by astrocytes, suggesting that proenkephalin or its derived peptides help to mediate region-specific brain functions.",

keywords = "Astrocyte culture, Cyclic AMP, Glial heterogeneity, Opiate peptide precursor, Preproenkephalin mRNA, mRNA regulation",

author = "Batter, {David K.} and Kessler, {John A.}",

year = "1991",

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T1 - Region-specific regulation of preproenkephalin mRNA in cultured astrocytes

AU - Batter, David K.

AU - Kessler, John A.

PY - 1991/8

Y1 - 1991/8

N2 - Regulation of preproenkephalin (PPE) mRNA was examined in astrocytes cultured from several regions of the neonatal rat brain. Astrocytes from these regions expressed differing levels of PPE mRNA, with higher levels in astrocytes from the hypothalamus followed by frontal cortex and striatum. Further, PPE mRNA was regulated differently in hypothalamic than in striatal glia. Treatment of striatal astrocytes with the β-adrenergic agonist, isoproterenol, or with agents which directly increased intracellular cAMP (forskolin or 8-bromo-cAMP) elevated levels of PPE mRNA. By contrast, none of these treatments altered levels of PPE mRNA in hypothalamic astrocytes despite increasing cAMP levels 60-fold. These observations indicate that there is striking regional heterogeneity in the expression and regulation of PPE mRNA by astrocytes, suggesting that proenkephalin or its derived peptides help to mediate region-specific brain functions.

AB - Regulation of preproenkephalin (PPE) mRNA was examined in astrocytes cultured from several regions of the neonatal rat brain. Astrocytes from these regions expressed differing levels of PPE mRNA, with higher levels in astrocytes from the hypothalamus followed by frontal cortex and striatum. Further, PPE mRNA was regulated differently in hypothalamic than in striatal glia. Treatment of striatal astrocytes with the β-adrenergic agonist, isoproterenol, or with agents which directly increased intracellular cAMP (forskolin or 8-bromo-cAMP) elevated levels of PPE mRNA. By contrast, none of these treatments altered levels of PPE mRNA in hypothalamic astrocytes despite increasing cAMP levels 60-fold. These observations indicate that there is striking regional heterogeneity in the expression and regulation of PPE mRNA by astrocytes, suggesting that proenkephalin or its derived peptides help to mediate region-specific brain functions.

KW - Astrocyte culture

KW - Cyclic AMP

KW - Glial heterogeneity

KW - Opiate peptide precursor

KW - Preproenkephalin mRNA

KW - mRNA regulation

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ER -

Region-specific regulation of preproenkephalin mRNA in cultured astrocytes

Abstract

Keywords

ASJC Scopus subject areas

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