Regional heterogeneity in expression of the sphingosine-1-phosphate pathway in the female rat lower urinary tract

Katherine S. Sandhu*, Rowena G. Chua, Xinhua Zhang, Nirmala Devi Kanika, Sarah A. Collins, Magdy Mikhail, Arnold Melman, Michael E. DiSanto

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Objective: We investigated the existence and regional distribution of sphingosine-1-phosphate regulatory enzymes and receptors in the lower urinary tract and determined the functional role of sphingosine-1-phosphate receptors in the bladder. Study Design: Lower urinary tract tissue from 10 female rats was harvested for real-time reverse transcriptase-polymerase chain reaction or organ bath physiology, whereas blood serum was obtained for high-performance liquid chromatography determination of sphingosine-1-phosphate levels. Statistical analysis included the Student t test and analysis of variance. Results: All 3 sphingosine-1-phosphate receptors and major enzymes were expressed throughout the lower urinary tract, but expression and physiologic force generation varied among regions. Sphingosine-1-phosphate was detected in serum. Conclusion: We provide novel data that the sphingosine-1-phosphate signaling pathway regulatory proteins exist throughout the female rat lower urinary tract, but that relative expression exhibits regional heterogeneity corresponding with lower urinary tract contractile response to sphingosine-1-phosphate. Our study suggests that sphingosine-1-phosphate signaling is important in the lower urinary tract and identifies this pathway as a possible target for altering bladder smooth muscle tone.

Original languageEnglish (US)
Pages (from-to)576.e1-576.e7
JournalAmerican journal of obstetrics and gynecology
Volume200
Issue number5
DOIs
StatePublished - May 2009

Keywords

  • G-protein-coupled receptor
  • overactive bladder
  • rho-kinase
  • smooth muscle
  • sphingosine-1-phosphate

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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