Regulation of adenylate cyclase activity mediated by muscarinic acetylcholine receptors

N. M. Nathanson, W. L. Klein, M. Nirenberg

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102 Scopus citations


Carbachol, an activator of muscarinic acetylcholine receptors of NG108-15 hybrid cells, inhibits adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC] rapidly and reversibly and slowly evokes a 200-300% increase in adenylate cyclase activity over a period of 24-30 hr. Both the inhibition of adenylate cyclase and the gradual increase in enzyme activity are dependent on muscarinic acetylcholine receptors and the receptor activator. Withdrawal of carbachol results in a gradual return of adenylate cyclase activity to control levels over a period of 6 hr; the half-life for decay of enzyme activity is 1.6 hr. These results show that muscarinic acetylcholine receptors mediate both transient and long-lived effects on adenylate cyclase activity that resemble those of opiates.

Original languageEnglish (US)
Pages (from-to)1788-1791
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number4
StatePublished - 1978

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