Abstract
We investigated the modulation of voltage dependent Ca2+ currents by chemokine receptors in heterologous expression systems and neurons. Fractalkine, SDF-1α, RANTES and MDC inhibited the IBa in CX3CR1-, CXCR4-, CCR5- and CCR4-expressing G1A1 cells, respectively. The IBa inhibition was voltage-dependent, exhibited prepulse facilitation, and was blocked by N-ethylmaleimide and pertussis toxin pretreatment, indicating that it was mediated by Gi/Go. Some chemokines also inhibited the IBa in subpopulations of dorsal root ganglion neurons and area postrema/nucleus tractus solitarius neurons. These data provide evidence that chemokines can potentially modulate neuronal signaling through the inhibition of neuronal Ca2+ currents.
Original language | English (US) |
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Pages (from-to) | 66-75 |
Number of pages | 10 |
Journal | Journal of Neuroimmunology |
Volume | 123 |
Issue number | 1-2 |
DOIs | |
State | Published - 2002 |
Funding
This work was supported by grants from the NIH DA02121, DA13141, MH40165, NS33826, DK44840 and NS21442 to RJM. SBO was supported in part by postdoctoral fellowships program from Korea Science and Engineering Foundation. We would like to thank Dr. Pat Gray of ICOS for the generous supply of CCR4 constructs and MDC used in these studies.
Keywords
- Chemokines
- Neuronal signaling
- Voltage-dependent Ca currents
ASJC Scopus subject areas
- Clinical Neurology
- Neurology
- Immunology and Allergy
- Immunology