@article{ae16ab1c2c3a4714aa8c233e5432d723,
title = "Regulation of histone acetylation and transcription by INHAT, a human cellular complex containing the set oncoprotein",
abstract = "Acetylation of histones by p300/CBP and PCAF is considered to be a critical step in transcriptional regulation. In order to understand the role of cellular activities that modulate histone acetylation and transcription, we have purified and characterized a multiprotein cellular complex that potently inhibits the histone acetyltransferase activity of p300/CBP and PCAF. We have mapped a novel acetyltransferase-inhibitory domain of this INHAT (inhibitor of acetyltransferases) complex that binds to histones and masks them from being acetyltransferase substrates. Endogenous INHAT subunits, which include the Set/TAF-Iβ oncoprotein, associate with chromatin in vivo and can block coactivator-mediated transcription when transfected in cells. We propose that histone masking by INHAT plays a regulatory role in chromatin modification and serves as a novel mechanism of transcriptional regulation.",
author = "Seo, {Sang beom} and Peter McNamara and Soyoung Heo and April Turner and Lane, {William S.} and Debabrata Chakravarti",
note = "Funding Information: We thank Drs. M. Lazar, G. FitzGerald, B. Forman, I. Schulman, and S. Ghosh for critical reading of the manuscript; and R. Evans for continuous support, helpful suggestions, and encouragement. We are grateful to Drs. K. Nagata for TAF-I α and Set/TAF-I β, and G. Pasternack for pp32 cDNA clones and antibodies; Y. Nakatani and V. Ogryzko for p300, p300 (964-1810), and PCAF baculoviruses; and G. Blobel for providing the recombinant GST-CBP derivative. We also thank Dr. P. Loll, A. Bevivino, and J. Kaplan for help during the the initial phase of INHAT purification, M. A. Leonard for artwork, K. A. Pierce for help with mass spectrometry, and the National Cell Culture Center (Minneapolis, MN) for large-scale propagation of HeLa cells used in this study. This work was supported by National Institutes of Health research grant (RO1-DK57079-01A1 to D. C.) and pilot project grants from the Diabetes Center (P30-DK19925) and the Cancer Center of the University of Pennsylvania.",
year = "2001",
month = jan,
day = "12",
doi = "10.1016/S0092-8674(01)00196-9",
language = "English (US)",
volume = "104",
pages = "119--130",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "1",
}