Regulation of human natural killing. I. The role of monocytes, interferon, and prostaglandins

H. S. Koren, S. J. Anderson, D. G. Fischer, C. S. Copeland, P. J. Jensen

Research output: Contribution to journalArticlepeer-review

129 Scopus citations

Abstract

We have found that human endogenous natural killer activity as measured in a rapid 51Cr release assay is unaffected by the presence of monocytes. Moreover, stimulation of natural killer cells by poly I:C is independent of monocytes. In contrast, the presence of monocytes in a mixed population of mononuclear cells that have been stimulated by poly I:C suppresses NK activity. The suppression is shown to be partially reversible if indomethacin (10 -6 M) is added to the cultures during stimulation. Culture supernatants of monocytes stimulated with poly I:C are shown to contain PGE 1 in a radioimmunoassay, and have NK inhibitory activity comparable to that obtained with exogenous PGE 1 added to NK assays at a concentration range of 10 -7 to 10 -9 M. Culture supernatants from poly I:C-stimulated monocytes do not have detectable levels of antiviral activity. In contrast, plastic nonadherent cells stimulated with poly I:C produce significant levels of interferon (100 to 200 U/ml/2 x 10 6 cells) but almost undetectable amounts of PGE. Supernatants of nonadherent cells incubated with indomethacin (10 -6 M) alone augment NK activity. Taken together, the results suggest that stimulation of mononuclear cells with poly I:C is dependent on and regulated by the relative levels of interferon produced by lymphocytes and PGE produced by monocytes.

Original languageEnglish (US)
Pages (from-to)2007-2013
Number of pages7
JournalJournal of Immunology
Volume127
Issue number5
StatePublished - 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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