Regulation of ICAM-1-mediated fibroblast-T cell reciprocal interaction: Implications for modulation of gut inflammation

A. Musso, T. P. Condon, G. A. West, C. De La Motte, S. A. Strong, A. D. Levine, C. F. Bennett, C. Fiocchi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Background and Aims: Immune-nonimmune cell interactions modulate mucosal immunity. We investigated the expression of adhesion molecules by intestinal fibroblasts, the effect of immune cell-derived factor on fibroblast binding of T cells, and the consequences of interfering with adhesion molecule expression on fibroblast-T cell interaction. Methods: Expression of fibroblast intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 surface and messenger RNA (mRNA) was measured before and after exposure to immune cell-derived supernatants. Fibroblasts were treated with antibodies to ICAM-1 or VCAM-1, or ICAM-1 antisense oligonucleotide Isis 2302, before a T-cell adhesion assay. Results: Fibroblast activation by immune cell-derived cytokines enhanced ICAM-1 and VCAM-1 surface expression and mRNA as well as adhesiveness fort cells. Blockade with neutralizing antibodies showed that binding was almost exclusively dependent on ICAM-1. Isis 2302 specifically reduced fibroblast ICAM-1 mRNA and dose-dependently inhibited ICAM-1 surface expression and T- cell binding. Conclusions: ICAM-1 is essential for intestinal fibroblast binding of T cells, a phenomenon that is efficiently and specifically disrupted by ICAM-1 antisense oligonucleotides. These observations emphasize the crucial regulatory role of fibroblasts in mucosal immunity and their potential as targets for therapeutic intervention in intestinal inflammation.

Original languageEnglish (US)
Pages (from-to)546-556
Number of pages11
Issue number3
StatePublished - 1999

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology


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