Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification

Lisa E. Hang; Christopher R. Lopez; Xianpeng Liu; Jaime M. Williams; Inn Chung; Lei Wei; Alison A. Bertuch; Xiaolan Zhao

doi:10.1074/jbc.M113.526178

Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification

Lisa E. Hang, Christopher R. Lopez, Xianpeng Liu, Jaime M. Williams, Inn Chung, Lei Wei, Alison A. Bertuch, Xiaolan Zhao^*

^*Corresponding author for this work

Surgery, Thoracic Surgery Division

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Background: The Ku70-Ku80 ring encloses DNA ends to enable telomere protection and DNA repair. Results: Conditional sumoylation of Ku70 is reduced by mutating C-terminal lysines and this mutant shows decreased DNA interaction, shortened telomeres, and altered DNA repair. Conclusion: Our analyses suggest that sumoylation modulates Ku function by enhancing its DNA association. Significance: Ku is regulated by Ku70 C-terminal lysines and SUMO modification.

Original language	English (US)
Pages (from-to)	10308-10317
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	289
Issue number	15
DOIs	https://doi.org/10.1074/jbc.M113.526178
State	Published - Apr 11 2014

ASJC Scopus subject areas

Molecular Biology
Biochemistry
Cell Biology

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title = "Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification",

abstract = "Background: The Ku70-Ku80 ring encloses DNA ends to enable telomere protection and DNA repair. Results: Conditional sumoylation of Ku70 is reduced by mutating C-terminal lysines and this mutant shows decreased DNA interaction, shortened telomeres, and altered DNA repair. Conclusion: Our analyses suggest that sumoylation modulates Ku function by enhancing its DNA association. Significance: Ku is regulated by Ku70 C-terminal lysines and SUMO modification.",

author = "Hang, {Lisa E.} and Lopez, {Christopher R.} and Xianpeng Liu and Williams, {Jaime M.} and Inn Chung and Lei Wei and Bertuch, {Alison A.} and Xiaolan Zhao",

year = "2014",

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doi = "10.1074/jbc.M113.526178",

language = "English (US)",

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AU - Hang, Lisa E.

AU - Lopez, Christopher R.

AU - Liu, Xianpeng

AU - Williams, Jaime M.

AU - Chung, Inn

AU - Wei, Lei

AU - Bertuch, Alison A.

AU - Zhao, Xiaolan

PY - 2014/4/11

Y1 - 2014/4/11

N2 - Background: The Ku70-Ku80 ring encloses DNA ends to enable telomere protection and DNA repair. Results: Conditional sumoylation of Ku70 is reduced by mutating C-terminal lysines and this mutant shows decreased DNA interaction, shortened telomeres, and altered DNA repair. Conclusion: Our analyses suggest that sumoylation modulates Ku function by enhancing its DNA association. Significance: Ku is regulated by Ku70 C-terminal lysines and SUMO modification.

AB - Background: The Ku70-Ku80 ring encloses DNA ends to enable telomere protection and DNA repair. Results: Conditional sumoylation of Ku70 is reduced by mutating C-terminal lysines and this mutant shows decreased DNA interaction, shortened telomeres, and altered DNA repair. Conclusion: Our analyses suggest that sumoylation modulates Ku function by enhancing its DNA association. Significance: Ku is regulated by Ku70 C-terminal lysines and SUMO modification.

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Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification

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