Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification

Lisa E. Hang, Christopher R. Lopez, Xianpeng Liu, Jaime M. Williams, Inn Chung, Lei Wei, Alison A. Bertuch, Xiaolan Zhao*

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: The Ku70-Ku80 ring encloses DNA ends to enable telomere protection and DNA repair. Results: Conditional sumoylation of Ku70 is reduced by mutating C-terminal lysines and this mutant shows decreased DNA interaction, shortened telomeres, and altered DNA repair. Conclusion: Our analyses suggest that sumoylation modulates Ku function by enhancing its DNA association. Significance: Ku is regulated by Ku70 C-terminal lysines and SUMO modification.

Original languageEnglish (US)
Pages (from-to)10308-10317
Number of pages10
JournalJournal of Biological Chemistry
Volume289
Issue number15
DOIs
StatePublished - Apr 11 2014

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Sumoylation
DNA Repair
Lysine
DNA
Telomere Shortening
Telomere
Repair

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hang, L. E., Lopez, C. R., Liu, X., Williams, J. M., Chung, I., Wei, L., ... Zhao, X. (2014). Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification. Journal of Biological Chemistry, 289(15), 10308-10317. https://doi.org/10.1074/jbc.M113.526178
Hang, Lisa E. ; Lopez, Christopher R. ; Liu, Xianpeng ; Williams, Jaime M. ; Chung, Inn ; Wei, Lei ; Bertuch, Alison A. ; Zhao, Xiaolan. / Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 15. pp. 10308-10317.
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abstract = "Background: The Ku70-Ku80 ring encloses DNA ends to enable telomere protection and DNA repair. Results: Conditional sumoylation of Ku70 is reduced by mutating C-terminal lysines and this mutant shows decreased DNA interaction, shortened telomeres, and altered DNA repair. Conclusion: Our analyses suggest that sumoylation modulates Ku function by enhancing its DNA association. Significance: Ku is regulated by Ku70 C-terminal lysines and SUMO modification.",
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Hang, LE, Lopez, CR, Liu, X, Williams, JM, Chung, I, Wei, L, Bertuch, AA & Zhao, X 2014, 'Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification', Journal of Biological Chemistry, vol. 289, no. 15, pp. 10308-10317. https://doi.org/10.1074/jbc.M113.526178

Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification. / Hang, Lisa E.; Lopez, Christopher R.; Liu, Xianpeng; Williams, Jaime M.; Chung, Inn; Wei, Lei; Bertuch, Alison A.; Zhao, Xiaolan.

In: Journal of Biological Chemistry, Vol. 289, No. 15, 11.04.2014, p. 10308-10317.

Research output: Contribution to journalArticle

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AU - Chung, Inn

AU - Wei, Lei

AU - Bertuch, Alison A.

AU - Zhao, Xiaolan

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AB - Background: The Ku70-Ku80 ring encloses DNA ends to enable telomere protection and DNA repair. Results: Conditional sumoylation of Ku70 is reduced by mutating C-terminal lysines and this mutant shows decreased DNA interaction, shortened telomeres, and altered DNA repair. Conclusion: Our analyses suggest that sumoylation modulates Ku function by enhancing its DNA association. Significance: Ku is regulated by Ku70 C-terminal lysines and SUMO modification.

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