Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification

Lisa E. Hang, Christopher R. Lopez, Xianpeng Liu, Jaime M. Williams, Inn Chung, Lei Wei, Alison A. Bertuch, Xiaolan Zhao*

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Background: The Ku70-Ku80 ring encloses DNA ends to enable telomere protection and DNA repair. Results: Conditional sumoylation of Ku70 is reduced by mutating C-terminal lysines and this mutant shows decreased DNA interaction, shortened telomeres, and altered DNA repair. Conclusion: Our analyses suggest that sumoylation modulates Ku function by enhancing its DNA association. Significance: Ku is regulated by Ku70 C-terminal lysines and SUMO modification.

Original languageEnglish (US)
Pages (from-to)10308-10317
Number of pages10
JournalJournal of Biological Chemistry
Volume289
Issue number15
DOIs
StatePublished - Apr 11 2014

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hang, L. E., Lopez, C. R., Liu, X., Williams, J. M., Chung, I., Wei, L., Bertuch, A. A., & Zhao, X. (2014). Regulation of Ku-DNA association by yku70 C-terminal tail and SUMO modification. Journal of Biological Chemistry, 289(15), 10308-10317. https://doi.org/10.1074/jbc.M113.526178