Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa

Everett C. Pesci, James P. Pearson, Patrick C. Seed, Barbara H. Iglewski*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

714 Scopus citations


The production of several virulence factors by Pseudomonas aeruginosa is controlled according to cell density through two quorum-sensing systems, las and rhl. The las system is comprised of the transcriptional activator protein LasR and of LasI, which directs the synthesis of the autoinducer PAI-1. Similarly, the rhl system consists of the transcriptional activator protein RhlR and of RhlI, which directs synthesis of the autoinducer PAI-2 (formerly referred to as factor 2). To study the interrelation between the two P. aeruginosa quorum-sensing systems, we fused a lacZ reporter gene to lasR, rhlR, and rhlA and monitored expression of these three genes under various conditions. Our data indicate that lasR and rhlR are expressed in a growth- dependent manner, with activation of each gene occurring during the last half of log-phase growth. We also show that the las quorum-sensing system controls the rhl quorum-sensing system in two ways. First, we found that LasR and PAl- 1 activated rhlR transcription. Second, we showed that PAI-1 blocked PAI-2 from binding to RhlR, thereby inhibiting the expression of rhlA. Our data thus indicate that the las system exerts two levels of control on RhlR, transcriptional and posttranslational.

Original languageEnglish (US)
Pages (from-to)3127-3132
Number of pages6
JournalJournal of bacteriology
Issue number10
StatePublished - May 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology


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