Regulation of mouse follicle development by follicle-stimulating hormone in a three-dimensional in vitro culture system is dependent on follicle stage and dose

Pamela K. Kreeger, Nisha N. Fernandes, Teresa K. Woodruff, Lonnie D. Shea*

*Corresponding author for this work

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

The developmental requirements of ovarian follicles are dependent on the maturation stage of the follicle; in particular, elegant studies with genetic models have indicated that FSH is required for antral, but not preantral, follicle growth and maturation. To elucidate further the role of FSH and other regulatory molecules in preantral follicle development, in vitro culture systems are needed. We employed a biomaterials-based approach to follicle culture, in which follicles were encapsulated within matrices that were tailored to the specific developmental needs of the follicle. This three-dimensional system was used to examine the impact of increasing doses of FSH on follicle development for two-layered secondary (100-130 μm; two layers of granulosa cells surrounding the oocyte) and multilayered secondary (150-180 μm, several layers of granulosa cells surrounding the oocyte) follicles isolated from mice. Two-layered secondary follicles were FSH responsive when cultured in alginate-collagen I matrices, exhibiting FSH dose-dependent increases in follicle growth, lactate production, and steroid secretion. Multilayered secondary follicles were FSH dependent, with follicle survival, growth, steroid secretion, metabolism, and oocyte maturation all regulated by FSH. However, doses greater than 25 mlU/ml of FSH negatively impacted multilayered secondary follicle development (reduced follicle survival). The present results indicate that the hormonal and environmental needs of the follicular complex change during the maturation process. The culture system can be adapted to each stage of development, which will be especially critical for translation to human follicles that have a longer developmental period.

Original languageEnglish (US)
Pages (from-to)942-950
Number of pages9
JournalBiology of reproduction
Volume73
Issue number5
DOIs
StatePublished - Nov 2005

Keywords

  • Follicle
  • Follicle-stimulating hormone
  • Follicular development
  • Oocyte development
  • Ovary

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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