Regulation of Muscarinic Acetylcholine Receptor Concentration in Cloned Neuroblastoma Cells

Gary S. Shifrin, William L Klein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Abstract: The concentration of muscarinic acetylcholine receptors in the neuroblastoma cell line NIE‐115 is regulated by receptor activation. Muscarinic agonists cause a time and dose‐dependent loss of [3H]quinuclidinyl benzilate binding sites from cultured cells. Muscarinic antagonists have no effect on receptor concentration and block agonist‐induced regulation. The maximum decrease in steady state receptor levels is 80% and occurs within 9 h. The altered steady state concentration persists as long as agonist remains present. Upon withdrawal of agonist, the concentration of receptors returns to control levels. This increase requires protein synthesis. Kinetically, the increase in receptors following withdrawal of agonist is slower than the decrease caused by addition of agonist, suggesting that bursts of receptor activation could lower receptor levels. In harmony with this prediction, cycles in which receptors are active for 15 min and then inactive for 15 min cause a 50% decrease in receptor concentration in a 6‐h period.

Original languageEnglish (US)
Pages (from-to)993-999
Number of pages7
JournalJournal of Neurochemistry
Volume34
Issue number4
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Cellular and Molecular Neuroscience

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