TY - JOUR
T1 - Regulation of protein kinase C inactivation by fas-associated protein with death domain
AU - Cheng, Wei
AU - Wang, Lu
AU - Zhang, Rong
AU - Du, Pan
AU - Yang, Bingya
AU - Zhuang, Hongqin
AU - Tang, Bo
AU - Yao, Chun
AU - Yu, Mei
AU - Wang, Yuxuan
AU - Zhang, Jing
AU - Yin, Wu
AU - Li, Jiahuang
AU - Zheng, Weijuan
AU - Lu, Min
AU - Hua, Zichun
PY - 2012/7/27
Y1 - 2012/7/27
N2 - Protein kinase C (PKC) plays important roles in diverse cellular processes. PKC has been implicated in regulating Fas-associated protein with death domain (FADD), an important adaptor protein involved in regulating death receptor-mediated apoptosis. FADD also plays an important role in non-apoptosis processes. The functional interaction of PKC and FADD in non-apoptotic processes has not been examined. In this study, we show thatFADDis involved in maintaining the phosphorylation of the turn motif and hydrophobic motif in the activated conventional PKC (cPKC). A phosphoryl-mimicking mutation (S191D) in FADD (FADD-D) abolished the function of FADD in the facilitation of the turn motif and hydrophobic motif dephosphorylation of cPKC, suggesting that phosphorylation of Ser-191 negatively regulates FADD.We show that FADD interacts with PP2A, which is a major phosphatase involved in dephosphorylation of activated cPKC and FADD deficiency abolished PP2A mediated dephosphorylation of cPKC. We show that FADD deficiency leads to increased stability and activity of cPKC, which, in turn, promotes cytoskeleton reorganization, cell motility, and chemotaxis. Collectively, these results reveal a novel function of FADD in a non-apoptotic process by modulating cPKC dephosphorylation, stability, and signaling termination.
AB - Protein kinase C (PKC) plays important roles in diverse cellular processes. PKC has been implicated in regulating Fas-associated protein with death domain (FADD), an important adaptor protein involved in regulating death receptor-mediated apoptosis. FADD also plays an important role in non-apoptosis processes. The functional interaction of PKC and FADD in non-apoptotic processes has not been examined. In this study, we show thatFADDis involved in maintaining the phosphorylation of the turn motif and hydrophobic motif in the activated conventional PKC (cPKC). A phosphoryl-mimicking mutation (S191D) in FADD (FADD-D) abolished the function of FADD in the facilitation of the turn motif and hydrophobic motif dephosphorylation of cPKC, suggesting that phosphorylation of Ser-191 negatively regulates FADD.We show that FADD interacts with PP2A, which is a major phosphatase involved in dephosphorylation of activated cPKC and FADD deficiency abolished PP2A mediated dephosphorylation of cPKC. We show that FADD deficiency leads to increased stability and activity of cPKC, which, in turn, promotes cytoskeleton reorganization, cell motility, and chemotaxis. Collectively, these results reveal a novel function of FADD in a non-apoptotic process by modulating cPKC dephosphorylation, stability, and signaling termination.
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U2 - 10.1074/jbc.M112.342170
DO - 10.1074/jbc.M112.342170
M3 - Article
C2 - 22582393
AN - SCOPUS:84864387916
SN - 0021-9258
VL - 287
SP - 26126
EP - 26135
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 31
ER -