Regulation of the translation initiation factor eIF4F by multiple mechanisms in human cytomegalovirus-infected cells

Derek Walsh, Cesar Perez, Joanna Notary, Ian Mohr*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

As a viral opportunistic pathogen associated with serious disease among the immunocompromisedl and congenital defects in newborns, human cytomegalovirus (HCMV) must engage the translational machinery within its host cell to synthesize the viral proteins required for its productive growth. However, unlike many viruses, HCMV does not suppress the translation of host polypeptides. Here, we examine how HCMV regulates the cellular cap recognition complex eIF4F, a critical component of the cellular translation initiation apparatus that recruits the 40S ribosome to the 5′ end of the mRNA. This study establishes that the cap binding protein eIF4E, together with the translational repressor 4E-BP1, are both phosphorylated early in the productive viral growth cycle and that the activity of the cellular eIF4E kinase, mnk, is critical for efficient viral replication. Furthermore, HCMV replication also induces an increase in the overall abundance of eIF4F components and promotes assembly of eIF4F complexes. Notably, increasing the abundance of select eIF4F core components and associated factors alters the ratio of active eIF4F complexes in relation to the 4E-BP1 translational represser, illustrating a new strategy through which members of the herpesvirus family enhance eIF4F activity during their replicative cycle.

Original languageEnglish (US)
Pages (from-to)8057-8064
Number of pages8
JournalJournal of virology
Volume79
Issue number13
DOIs
StatePublished - Jul 2005

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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