Relationship of a leukemia-associated antigen to the presence of lymphoblasts in the peripheral blood in children with acute lymphocytic leukemia

Elaine R Morgan, C. C.S. Hsu

Research output: Contribution to journalArticlepeer-review

Abstract

Peripheral blood samples from 57 children with newly diagnosed E-rosette-negative, surface-immunoglobulin-negative acute lymphocytic leukemia (ALL) were studied for the presence of a leukemia-associated antigen (ALLA). Ficoll-Hypaque separated cells were tested using a rabbit antiserum to human null lymphoblasts and an indirect immunofluorescent assay. The percentage of ALLA-positive cells was compared to the percentage of lymphoblasts determined by differential counts of a Wright-Giemsa-stained smear of a concurrently obtained peripheral blood sample. The mean ratio of percentage of lymphoblasts to percentage of ALLA-positive cells was 0.90. However, in 13 patients, the ratio of percent of ALLA-positive cells to percent of lymphoblasts was equal to or greater than 2:1. In the blood of 6 additional children (5 newly diagnosed, 1 relapsed patient) in whom no morphologically identifiable lymphoblasts were detected, ALLA-positive cells were present (7%-49%). These results indicate that testing for ALLA-positive cells is a sensitive technique for detection of leukemic cells in children with ALLA-positive ALL.

Original languageEnglish (US)
Pages (from-to)879-882
Number of pages4
JournalBlood
Volume57
Issue number5
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

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