Reliability of estimates of diploid human spermatozoa using multicolour fluorescence in-situ hybridization

Alfred Rademaker, Elizabeth Spriggs, Evelyn Ko, Renée Martin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Multicolour fluorescence in-situ hybridization (FISH) analysis permits distinction between disomic and diploid spermatozoa. Thus estimates of the frequency of diploid spermatozoa can be obtained for human semen samples. The issue of the accuracy and reliability of these diploidy estimates has been addressed by analysing diploidy frequencies in 10 men using the same sperm sample to estimate diploidy twice - once during two-colour FISH analysis of disomy for chromosomes 1 and 12 and a second independent analysis of three-colour FISH for disomy estimates for chromosomes X and Y (with chromosome 1 used as the autosomal control). A minimum of 10,000 spermatozoa per hybridization per male was counted for a total of over 200,000 spermatozoa analysed. The mean frequency of diploid spermatozoa was 0.13% for the autosomal study and 0.14% for the sex chromosomal study, which were not significantly different. One donor had extremely divergent values of diploidy in the two studies. Analysis of values in the other nine donors demonstrated no significant difference in the two diploidy estimates. These results indicate that the FISH technique is an accurate and reliable method for determining diploid frequencies in human spermatozoa.

Original languageEnglish (US)
Pages (from-to)77-79
Number of pages3
JournalHuman Reproduction
Volume12
Issue number1
DOIs
StatePublished - Jan 1997

Keywords

  • Chromosome abnormalities
  • Diploidy frequency
  • Fluorescence in-situ hybridization
  • Spermatozoa

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Fingerprint Dive into the research topics of 'Reliability of estimates of diploid human spermatozoa using multicolour fluorescence in-situ hybridization'. Together they form a unique fingerprint.

Cite this