Remodeling Characteristics and Collagen Distributions of Biologic Scaffold Materials Biopsied From Postmastectomy Breast Reconstruction Sites

Jaime A. Cavallo, Noopur Gangopadhyay, Jason Dudas, Andres A. Roma, Mateusz S. Jasielec, Jack Baty, Sara Baalman, Margaret M. Frisella, Marissa M. Tenenbaum, Terence M. Myckatyn, Brent D. Matthews, Corey R. Deeken*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Objective: The study purpose was to evaluate the associations between patient characteristics and the histologic remodeling scores of acellular dermal matrices (ADMs) biopsied from breast reconstruction sites in the first attempt to generate a multivariable risk prediction model of nonconstructive remodeling. It was hypothesized that host characteristics and surgical site assessments predict the degree of graft remodeling for ADMs used during breast reconstruction. Methods: The ADMs were biopsied from the breast reconstruction sites of n = 62 patients during a subsequent breast procedure, stained with hematoxylin-eosin, and evaluated according to a semi-quantitative scoring system for remodeling characteristics (cell types, cell infiltration, extracellular matrix deposition, scaffold degradation, fibrous encapsulation, and neovascularization) and a mean composite score. Biopsies were stained with Sirius Red and Fast Green, and analyzed to determine the collagen I:III ratio. Based on univariate analyses between subject clinical characteristics and the histologic remodeling scores, cohort variables were selected for multivariable regression models using a P value of 0.20 or less. Results: The composite score model yielded 3 variables: pack-year history, corticosteroid use, and radiation timing (rpseudo2 = 0.81). The model for collagen I yielded 2 variables: corticosteroid use and reason for reoperation (rpseudo2 = 0.78). The model for collagen III yielded 1 variable: reason for reoperation (rpseudo2 = 0.35). Conclusions: These preliminary results constitute the first steps in generating a risk prediction model that predicts the patients and clinical circumstances most likely to experience nonconstructive remodeling of biologic grafts used to reconstruct the breast.

Original languageEnglish (US)
Pages (from-to)74-83
Number of pages10
JournalAnnals of plastic surgery
Volume75
Issue number1
DOIs
StatePublished - Jul 25 2015

Keywords

  • Sirius Red/Fast Green
  • acellular dermal matrix
  • biologic scaffold
  • breast augmentation
  • breast reconstruction
  • human dermis
  • soft tissue repair
  • tissue remodeling
  • type I collagen
  • type III collagen

ASJC Scopus subject areas

  • Surgery

Fingerprint

Dive into the research topics of 'Remodeling Characteristics and Collagen Distributions of Biologic Scaffold Materials Biopsied From Postmastectomy Breast Reconstruction Sites'. Together they form a unique fingerprint.

Cite this