TY - JOUR
T1 - Remote Control of Heterodimeric Magnetic Nanoswitch Regulates the Adhesion and Differentiation of Stem Cells
AU - Kang, Heemin
AU - Jung, Hee Joon
AU - Wong, Dexter Siu Hong
AU - Kim, Sung Kyu
AU - Lin, Sien
AU - Chan, Kai Fung
AU - Zhang, Li
AU - Li, Gang
AU - Dravid, Vinayak P.
AU - Bian, Liming
N1 - Funding Information:
Project 31570979 is supported by the National Natural Science Foundation of China. This work is supported by a General Research Fund grant from the Research Grants Council of Hong Kong (project no. 14202215 and 14220716); the Health and Medical Research Fund the Food and Health Bureau, the Government of the Hong Kong Special Administrative Region (reference no. 04152836 and 03140056); the project BME-p3-15 of the Shun Hing Institute of Advanced Engineering, The Chinese University of Hong Kong; the Chow Yuk Ho Technology Centre for Innovative Medicine, The Chinese University of Hong Kong. This work made use of the EPIC facility of Northwestern University's NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205); the MRSEC program (NSF DMR-1720139) at the Materials Research Center; the International Institute for Nanotechnology (IIN); the Keck Foundation; and the State of Illinois, through the IIN.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/5/9
Y1 - 2018/5/9
N2 - Remote, noninvasive, and reversible control over the nanoscale presentation of bioactive ligands, such as Arg-Gly-Asp (RGD) peptide, is highly desirable for temporally regulating cellular functions in vivo. Herein, we present a novel strategy for physically uncaging RGD using a magnetic field that allows safe and deep tissue penetration. We developed a heterodimeric nanoswitch consisting of a magnetic nanocage (MNC) coupled to an underlying RGD-coated gold nanoparticle (AuNP) via a long flexible linker. Magnetically controlled movement of MNC relative to AuNP allowed reversible uncaging and caging of RGD that modulate physical accessibility of RGD for integrin binding, thereby regulating stem cell adhesion, both in vitro and in vivo. Reversible RGD uncaging by the magnetic nanoswitch allowed temporal regulation of stem cell adhesion, differentiation, and mechanosensing. This physical and reversible RGD uncaging utilizing heterodimeric magnetic nanoswitch is unprecedented and holds promise in the remote control of cellular behaviors in vivo.
AB - Remote, noninvasive, and reversible control over the nanoscale presentation of bioactive ligands, such as Arg-Gly-Asp (RGD) peptide, is highly desirable for temporally regulating cellular functions in vivo. Herein, we present a novel strategy for physically uncaging RGD using a magnetic field that allows safe and deep tissue penetration. We developed a heterodimeric nanoswitch consisting of a magnetic nanocage (MNC) coupled to an underlying RGD-coated gold nanoparticle (AuNP) via a long flexible linker. Magnetically controlled movement of MNC relative to AuNP allowed reversible uncaging and caging of RGD that modulate physical accessibility of RGD for integrin binding, thereby regulating stem cell adhesion, both in vitro and in vivo. Reversible RGD uncaging by the magnetic nanoswitch allowed temporal regulation of stem cell adhesion, differentiation, and mechanosensing. This physical and reversible RGD uncaging utilizing heterodimeric magnetic nanoswitch is unprecedented and holds promise in the remote control of cellular behaviors in vivo.
UR - http://www.scopus.com/inward/record.url?scp=85046413596&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046413596&partnerID=8YFLogxK
U2 - 10.1021/jacs.8b03001
DO - 10.1021/jacs.8b03001
M3 - Article
C2 - 29681155
AN - SCOPUS:85046413596
SN - 0002-7863
VL - 140
SP - 5909
EP - 5913
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 18
ER -