Renal acid excretion and intracellular pH in salt-sensitive genetic hypertension

Daniel C. Batlle*, Arya M. Sharma, Mouhamed W. Alsheikha, Maria Sobrero, Abdulkarim Saleh, Cory Gutterman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


Acid-base status and renal acid excretion were studied in the Dahl/Rapp salt-sensitive (S) rat and its genetically salt-resistant counterpart (R). S rats developed hypertension while on a very high salt diet (8%) and while on a more physiological salt diet (1%) and remained normotensive while on a very low salt diet (0.08%). Under the high salt diet, intracellular pH measured in freshly isolated thymic lymphocytes using 2′,7′-bis (carboxyethyl)-5 (6)-carboxyfluorescein acetomethyl ester, a pH-sensitive dye, was lower in S than in R rats both when measured in the presence of HCO3/CO2 (7.32±0.02 vs. 7.38±0.02, respectively, P < 0.05) and in its absence (7.18±0.04 vs. 7.27±0.02, respectively, P < 0.05). Under the high salt diet, net acid excretion was higher in S than R rats (1,777±111 vs. 1,017±73 μEq/24 h per 100 g body wt, respectively, P <0.001), and this difference was due to higher rates of both titratable acid and ammonium excretion. Directionally similar differences in intracellular pH and net acid excretion between S and R rats were also observed in salt-restricted animals. In S and R rats placed on a normal salt intake (1%) and strictly pair-fed to control food intake as a determinant of dietary acid, net acid excretion was also higher in S than in R rats (562±27 vs. 329±21 μEq/24 h per 100 g, respectively, P <0.01). No significant difference in either blood pH or bicarbonate levels were found between S and R rats on either the 0.08%, 1%, or 8% salt diets. We conclude that renal acid excretion is augmented in the salt-sensitive Dahl/Rapp rat. Enhanced renal acid excretion may be a marker of increased acid production by cells from subjects with salt-sensitive hypertension.

Original languageEnglish (US)
Pages (from-to)2178-2184
Number of pages7
JournalJournal of Clinical Investigation
Issue number5
StatePublished - May 1993


  • Acid base
  • Acid excretion
  • Intracellular pH
  • Na/H antiporter
  • Salt-sensitive hypertension

ASJC Scopus subject areas

  • General Medicine


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