Abstract
An in vitro selection method based on the autolytic cleavage of yeast tRNAPhe by Pb2+ was applied to obtain tRNA derivatives with the anticodon hairpin replaced by four single-stranded nucleotides. Based on the rates of the site-specific cleavage by Pb2+ and the presence of a specific UV-induced crosslink, certain tetranucieotide sequences allow proper folding of the rest of the tRNA molecule, wheras others do not. One such successful tetramer sequence was also used to replace the acceptor stem of yeast tRNAPhe and the anticodon hairpin of E.coli tRNAPhe without disrupting folding. These experiments suggest that certain tetramers may be able to replace structurally non essential hairpins in any RNA.
Original language | English (US) |
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Pages (from-to) | 531-535 |
Number of pages | 5 |
Journal | Nucleic acids research |
Volume | 21 |
Issue number | 3 |
DOIs | |
State | Published - Feb 11 1993 |
Funding
This work was supported by N1H grant GM 37552. B.D. was partially supported by Deutscher Akademischer Austauschdienst (DAAD). T.P. acknowledges a postdoctoral fellowship from Damon Runyon-Walter Winchell Cancer Research Fund (DRG-1103). We thank J.Doudna and M.Stubenrauch for their comments on the manuscript. We also thank the W.M.Keck Foundation for their generous support of RNA science on the Boulder campus.
ASJC Scopus subject areas
- Genetics