Replacement of RNA hairpins by in vitro selected tetranucleotides

Bernhard Dichtl, Tao Pan, Anthony B. Direnzo, Olke C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

An in vitro selection method based on the autolytic cleavage of yeast tRNAPhe by Pb2+ was applied to obtain tRNA derivatives with the anticodon hairpin replaced by four single-stranded nucleotides. Based on the rates of the site-specific cleavage by Pb2+ and the presence of a specific UV-induced crosslink, certain tetranucieotide sequences allow proper folding of the rest of the tRNA molecule, wheras others do not. One such successful tetramer sequence was also used to replace the acceptor stem of yeast tRNAPhe and the anticodon hairpin of E.coli tRNAPhe without disrupting folding. These experiments suggest that certain tetramers may be able to replace structurally non essential hairpins in any RNA.

Original languageEnglish (US)
Pages (from-to)531-535
Number of pages5
JournalNucleic acids research
Volume21
Issue number3
DOIs
StatePublished - Feb 11 1993

ASJC Scopus subject areas

  • Genetics

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