Abstract
Procedures for the isolation and in vitro maintenance of oval (periductular) cells obtained from pancreas of rats maintained on copper-deficient diet for 8 wk are described. Oval cells grew readily in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, and have been continuously propagated in culture for 6 mo. through 10 passages. Morphologically, these cells showed epithelial features characterized by frequent cell junctions, microvilli, intermediate filaments, and attempted lumen formation. These cells were negative for y-glutamyltranspeptidase but stained positively with OV-6 antibodies. Chromosomal analysis revealed hyperdiploid pattern with a model number of 68. These pancreatic oval cells of rat pancreas should serve as a valuable tool for establishing subcloned cell lines for the study of transdifferentiation and in establishing the stem-cell potential.
Original language | English (US) |
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Pages (from-to) | 121-124 |
Number of pages | 4 |
Journal | Journal of Tissue Culture Methods |
Volume | 13 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1991 |
Keywords
- autoradiography
- ductular cells
- hepatocytes
- transdifferentiation
ASJC Scopus subject areas
- Anatomy
- Pathology and Forensic Medicine