Replicative culture in vitro of pancreatic epithelial oval cells derived from rats after copper deficiency-induced acinar cell depletion

Sambasiva Rao Musunuri*, Janardan K Reddy

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Procedures for the isolation and in vitro maintenance of oval (periductular) cells obtained from pancreas of rats maintained on copper-deficient diet for 8 wk are described. Oval cells grew readily in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, and have been continuously propagated in culture for 6 mo. through 10 passages. Morphologically, these cells showed epithelial features characterized by frequent cell junctions, microvilli, intermediate filaments, and attempted lumen formation. These cells were negative for y-glutamyltranspeptidase but stained positively with OV-6 antibodies. Chromosomal analysis revealed hyperdiploid pattern with a model number of 68. These pancreatic oval cells of rat pancreas should serve as a valuable tool for establishing subcloned cell lines for the study of transdifferentiation and in establishing the stem-cell potential.

Original languageEnglish (US)
Pages (from-to)121-124
Number of pages4
JournalJournal of Tissue Culture Methods
Volume13
Issue number2
DOIs
StatePublished - Jun 1 1991

Keywords

  • autoradiography
  • ductular cells
  • hepatocytes
  • transdifferentiation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

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