Reporter constructs with low background activity utilizing the cat gene

Michael Boshart, Michael Klüppel, Andrea Schmidt, Günther Schütz*, Bruno Luckow

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

229 Scopus citations

Abstract

Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements within the vector.

Original languageEnglish (US)
Pages (from-to)129-130
Number of pages2
JournalGene
Volume110
Issue number1
DOIs
StatePublished - Jan 2 1992

Keywords

  • Recombinant DNA
  • chloramphenicol acetyltransferase assay
  • cryptic promoter/enhancer
  • gene transfer
  • promoter/enhancer analysis
  • transcriptional regulation

ASJC Scopus subject areas

  • Genetics

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