Reporter constructs with low background activity utilizing the cat gene

Michael Boshart; Michael Klüppel; Andrea Schmidt; Günther Schütz; Bruno Luckow

doi:10.1016/0378-1119(92)90456-Y

Reporter constructs with low background activity utilizing the cat gene

Michael Boshart, Michael Klüppel, Andrea Schmidt, Günther Schütz^*, Bruno Luckow

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

229 Scopus citations

Abstract

Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements within the vector.

Original language	English (US)
Pages (from-to)	129-130
Number of pages	2
Journal	Gene
Volume	110
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(92)90456-Y
State	Published - Jan 2 1992

Keywords

Recombinant DNA
chloramphenicol acetyltransferase assay
cryptic promoter/enhancer
gene transfer
promoter/enhancer analysis
transcriptional regulation

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(92)90456-Y

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title = "Reporter constructs with low background activity utilizing the cat gene",

abstract = "Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements within the vector.",

keywords = "Recombinant DNA, chloramphenicol acetyltransferase assay, cryptic promoter/enhancer, gene transfer, promoter/enhancer analysis, transcriptional regulation",

author = "Michael Boshart and Michael Kl{\"u}ppel and Andrea Schmidt and G{\"u}nther Sch{\"u}tz and Bruno Luckow",

year = "1992",

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doi = "10.1016/0378-1119(92)90456-Y",

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AU - Boshart, Michael

AU - Klüppel, Michael

AU - Schmidt, Andrea

AU - Schütz, Günther

AU - Luckow, Bruno

PY - 1992/1/2

Y1 - 1992/1/2

N2 - Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements within the vector.

AB - Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. These plasmids minimize the background of transcription derived from cryptic promoters or cryptic regulatory elements within the vector.

KW - Recombinant DNA

KW - chloramphenicol acetyltransferase assay

KW - cryptic promoter/enhancer

KW - gene transfer

KW - promoter/enhancer analysis

KW - transcriptional regulation

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JO - Gene

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