Reproducibility of endothelial microparticles in children and adolescents

Elise F. Northrop; Liming C. Milbauer; Kyle D. Rudser; Claudia K. Fox; Anna N. Solovey; Alexander M. Kaizer; Robert P. Hebbel; Aaron S. Kelly; Justin R. Ryder

doi:10.2217/bmm-2019-0229

Reproducibility of endothelial microparticles in children and adolescents

Elise F. Northrop, Liming C. Milbauer, Kyle D. Rudser, Claudia K. Fox, Anna N. Solovey, Alexander M. Kaizer, Robert P. Hebbel, Aaron S. Kelly, Justin R. Ryder^*

^*Corresponding author for this work

Surgery

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Aim: We assessed reproducibility of endothelial microparticles (EMPs) enumeration among youth. Methods & results: Four microparticle (MP) indices - total MP per microliter platelet free plasma (PFP), total EMPs per microliter PFP, percent activated EMPs and percent lactadherin positive (LACT[+]) of total EMPs - were measured at two visits (baseline and 7 ± 3 days follow-up) to determine reproducibility overall and by obesity status. We examined CD31⁺ or CD144⁺ with CD41-EMP events of size 0.3-1.0 μm. No statistically significant differences were observed between visits for any of the four MP indices. The within-participant and between-participant coefficient of variation was acceptable (range: 1.13-2.37) with good intraclass-correlation coefficient for all indices except total MP per microliter (range: 0.10-1.00). Conclusion: Total EMPs per microliter PFP, percent-activated EMPs and percent LACT(+) of total EMPs are reproducible among youth.

Original language	English (US)
Pages (from-to)	43-51
Number of pages	9
Journal	Biomarkers in Medicine
Volume	14
Issue number	1
DOIs	https://doi.org/10.2217/bmm-2019-0229
State	Published - 2019

Keywords

biomarkers
endothelial
microparticles
obesity
reproducibility
youth

ASJC Scopus subject areas

Drug Discovery
Biochemistry, medical
Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/bmm-2019-0229

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@article{978cc1094f4b47a59635aa8d72e7f526,

title = "Reproducibility of endothelial microparticles in children and adolescents",

abstract = "Aim: We assessed reproducibility of endothelial microparticles (EMPs) enumeration among youth. Methods & results: Four microparticle (MP) indices - total MP per microliter platelet free plasma (PFP), total EMPs per microliter PFP, percent activated EMPs and percent lactadherin positive (LACT[+]) of total EMPs - were measured at two visits (baseline and 7 ± 3 days follow-up) to determine reproducibility overall and by obesity status. We examined CD31+ or CD144+ with CD41-EMP events of size 0.3-1.0 μm. No statistically significant differences were observed between visits for any of the four MP indices. The within-participant and between-participant coefficient of variation was acceptable (range: 1.13-2.37) with good intraclass-correlation coefficient for all indices except total MP per microliter (range: 0.10-1.00). Conclusion: Total EMPs per microliter PFP, percent-activated EMPs and percent LACT(+) of total EMPs are reproducible among youth.",

keywords = "biomarkers, endothelial, microparticles, obesity, reproducibility, youth",

author = "Northrop, {Elise F.} and Milbauer, {Liming C.} and Rudser, {Kyle D.} and Fox, {Claudia K.} and Solovey, {Anna N.} and Kaizer, {Alexander M.} and Hebbel, {Robert P.} and Kelly, {Aaron S.} and Ryder, {Justin R.}",

note = "Funding Information: AS Kelly has served on pediatric obesity advisory boards (Novo Nordisk; Orexigen and Vivus) and serves as the signatory author for a pediatric obesity clinical trial sponsored by Novo Nordisk but does not receive personal or professional income for his services. AS Kelly receives research support (drug/placebo) from Astra Zeneca. JR Ryder receives research support (drug/placebo) from Boehringer Ingelheim. This study was supported by the NIH (grant number: 5R01HL110957). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2019 Future Medicine Ltd.",

year = "2019",

doi = "10.2217/bmm-2019-0229",

language = "English (US)",

volume = "14",

pages = "43--51",

journal = "Biomarkers in Medicine",

issn = "1752-0363",

publisher = "Future Medicine Ltd.",

number = "1",

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TY - JOUR

T1 - Reproducibility of endothelial microparticles in children and adolescents

AU - Northrop, Elise F.

AU - Milbauer, Liming C.

AU - Rudser, Kyle D.

AU - Fox, Claudia K.

AU - Solovey, Anna N.

AU - Kaizer, Alexander M.

AU - Hebbel, Robert P.

AU - Kelly, Aaron S.

AU - Ryder, Justin R.

N1 - Funding Information: AS Kelly has served on pediatric obesity advisory boards (Novo Nordisk; Orexigen and Vivus) and serves as the signatory author for a pediatric obesity clinical trial sponsored by Novo Nordisk but does not receive personal or professional income for his services. AS Kelly receives research support (drug/placebo) from Astra Zeneca. JR Ryder receives research support (drug/placebo) from Boehringer Ingelheim. This study was supported by the NIH (grant number: 5R01HL110957). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: © 2019 Future Medicine Ltd.

PY - 2019

Y1 - 2019

N2 - Aim: We assessed reproducibility of endothelial microparticles (EMPs) enumeration among youth. Methods & results: Four microparticle (MP) indices - total MP per microliter platelet free plasma (PFP), total EMPs per microliter PFP, percent activated EMPs and percent lactadherin positive (LACT[+]) of total EMPs - were measured at two visits (baseline and 7 ± 3 days follow-up) to determine reproducibility overall and by obesity status. We examined CD31+ or CD144+ with CD41-EMP events of size 0.3-1.0 μm. No statistically significant differences were observed between visits for any of the four MP indices. The within-participant and between-participant coefficient of variation was acceptable (range: 1.13-2.37) with good intraclass-correlation coefficient for all indices except total MP per microliter (range: 0.10-1.00). Conclusion: Total EMPs per microliter PFP, percent-activated EMPs and percent LACT(+) of total EMPs are reproducible among youth.

AB - Aim: We assessed reproducibility of endothelial microparticles (EMPs) enumeration among youth. Methods & results: Four microparticle (MP) indices - total MP per microliter platelet free plasma (PFP), total EMPs per microliter PFP, percent activated EMPs and percent lactadherin positive (LACT[+]) of total EMPs - were measured at two visits (baseline and 7 ± 3 days follow-up) to determine reproducibility overall and by obesity status. We examined CD31+ or CD144+ with CD41-EMP events of size 0.3-1.0 μm. No statistically significant differences were observed between visits for any of the four MP indices. The within-participant and between-participant coefficient of variation was acceptable (range: 1.13-2.37) with good intraclass-correlation coefficient for all indices except total MP per microliter (range: 0.10-1.00). Conclusion: Total EMPs per microliter PFP, percent-activated EMPs and percent LACT(+) of total EMPs are reproducible among youth.

KW - biomarkers

KW - endothelial

KW - microparticles

KW - obesity

KW - reproducibility

KW - youth

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U2 - 10.2217/bmm-2019-0229

DO - 10.2217/bmm-2019-0229

M3 - Article

C2 - 31729246

AN - SCOPUS:85077482948

SN - 1752-0363

VL - 14

SP - 43

EP - 51

JO - Biomarkers in Medicine

JF - Biomarkers in Medicine

IS - 1

ER -

Reproducibility of endothelial microparticles in children and adolescents

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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