Reprogramming factor stoichiometry influences the epigenetic state and biological properties of induced pluripotent stem cells

Bryce W. Carey, Styliani Markoulaki, Jacob H. Hanna, Dina A. Faddah, Yosef Buganim, Jongpil Kim, Kibibi Ganz, Eveline J. Steine, John P. Cassady, Menno P. Creyghton, G. Grant Welstead, Qing Gao, Rudolf Jaenisch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

265 Scopus citations

Abstract

We compared two genetically highly defined transgenic systems to identify parameters affecting reprogramming of somatic cells to a pluripotent state. Our results demonstrate that the level and stoichiometry of reprogramming factors during the reprogramming process strongly influence the resulting pluripotency of iPS cells. High expression of Oct4 and Klf4 combined with lower expression of c-Myc and Sox2 produced iPS cells that efficiently generated "all-iPSC mice" by tetraploid (4n) complementation, maintained normal imprinting at the Dlk1-Dio3 locus, and did not create mice with tumors. Loss of imprinting (LOI) at the Dlk1-Dio3 locus did not strictly correlate with reduced pluripotency though the efficiency of generating "all-iPSC mice" was diminished. Our data indicate that stoichiometry of reprogramming factors can influence epigenetic and biological properties of iPS cells. This concept complicates efforts to define a "generic" epigenetic state of iPSCs and ESCs and should be considered when comparing different iPS and ES cell lines.

Original languageEnglish (US)
Pages (from-to)588-598
Number of pages11
JournalCell stem cell
Volume9
Issue number6
DOIs
StatePublished - Dec 2 2011

ASJC Scopus subject areas

  • Genetics
  • Molecular Medicine
  • Cell Biology

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