Requirement of Src-family tyrosine kinases in fat accumulation

Yutong Sun, Yong Chao Ma, Jianyun Huang, Krystina Y. Chen, Deirdre K. McGarrigle, Xin Yun Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Src-family tyrosine kinases mediate many receptor signals to various biological responses. Here we investigate the requirement of Src-family tyrosine kinases in adipogenesis. The biochemical mechanism by which insulin induces adipogenesis, converting fibroblast cells to adipocytes, is not clear. We show that fibroblast cells deficient of three ubiquitously expressed Src-family members (Src, Yes, and Fyn), SYF cells, are refractory to hormonally induced fat accumulation. The defect is rescued by reintroduction of c-Src into SYF cells. Furthermore, Src-family tyrosine kinases are required in the early steps of insulin signaling; it is responsible for the tyrosine phosphorylation of adaptor protein c-Cbl. Deficiency of c-Cbl blocked adipogenesis. These genetic and biochemical data clearly demonstrate that Src-family tyrosine kinases serve as a critical signal relay, via phosphorylation of c-Cbl, for fat accumulation, and provide potential new strategies for treating obesity.

Original languageEnglish (US)
Pages (from-to)14455-14462
Number of pages8
JournalBiochemistry
Volume44
Issue number44
DOIs
StatePublished - Nov 8 2005

ASJC Scopus subject areas

  • Biochemistry

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