Resistance to linezolid: Characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci

J. Prystowsky, F. Siddiqui, J. Chosay, D. L. Shinabarger, J. Millichap, L. R. Peterson, G. A. Noskin*

*Corresponding author for this work

Research output: Contribution to journalArticle

252 Scopus citations

Abstract

To assess the potential for emergence of resistance during the use of linezolid, we tested 10 clinical isolates of vancomycin-resistant enterococci (VRE) (four Enterococcus faecalis, five Enterococcus faecium, and one Enterococcus gallinarum) as well as a vancomycin-susceptible control (ATCC 29212) strain of E. faecalis. The enterococci were exposed to doubling dilutions of linezolid for 12 passes. After the final passage, the linezolid plate growing VRE contained a higher drug concentration with E, faecalis than with E. faecium. DNA sequencing of the 23S rRNA genes revealed that linezolid resistance in three E. faecalis isolates was associated with a guanine to uracil transversion at bp 2576, while the one E. faecium isolate for which the MIC was 16 μg/ml contained a guanine to adenine transition at bp 2505.

Original languageEnglish (US)
Pages (from-to)2154-2156
Number of pages3
JournalAntimicrobial agents and chemotherapy
Volume45
Issue number7
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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