TY - JOUR
T1 - Resistance to linezolid
T2 - Characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci
AU - Prystowsky, J.
AU - Siddiqui, F.
AU - Chosay, J.
AU - Shinabarger, D. L.
AU - Millichap, J.
AU - Peterson, L. R.
AU - Noskin, G. A.
PY - 2001
Y1 - 2001
N2 - To assess the potential for emergence of resistance during the use of linezolid, we tested 10 clinical isolates of vancomycin-resistant enterococci (VRE) (four Enterococcus faecalis, five Enterococcus faecium, and one Enterococcus gallinarum) as well as a vancomycin-susceptible control (ATCC 29212) strain of E. faecalis. The enterococci were exposed to doubling dilutions of linezolid for 12 passes. After the final passage, the linezolid plate growing VRE contained a higher drug concentration with E, faecalis than with E. faecium. DNA sequencing of the 23S rRNA genes revealed that linezolid resistance in three E. faecalis isolates was associated with a guanine to uracil transversion at bp 2576, while the one E. faecium isolate for which the MIC was 16 μg/ml contained a guanine to adenine transition at bp 2505.
AB - To assess the potential for emergence of resistance during the use of linezolid, we tested 10 clinical isolates of vancomycin-resistant enterococci (VRE) (four Enterococcus faecalis, five Enterococcus faecium, and one Enterococcus gallinarum) as well as a vancomycin-susceptible control (ATCC 29212) strain of E. faecalis. The enterococci were exposed to doubling dilutions of linezolid for 12 passes. After the final passage, the linezolid plate growing VRE contained a higher drug concentration with E, faecalis than with E. faecium. DNA sequencing of the 23S rRNA genes revealed that linezolid resistance in three E. faecalis isolates was associated with a guanine to uracil transversion at bp 2576, while the one E. faecium isolate for which the MIC was 16 μg/ml contained a guanine to adenine transition at bp 2505.
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U2 - 10.1128/AAC.45.7.2154-2156.2001
DO - 10.1128/AAC.45.7.2154-2156.2001
M3 - Article
C2 - 11408243
AN - SCOPUS:0034962929
SN - 0066-4804
VL - 45
SP - 2154
EP - 2156
JO - Antimicrobial agents and chemotherapy
JF - Antimicrobial agents and chemotherapy
IS - 7
ER -