Resolving Single-Nanoconstruct Dynamics during Targeting and Nontargeting Live-Cell Membrane Interactions

Debanjan Bhowmik; Kayla S.B. Culver; Tingting Liu; Teri W. Odom

doi:10.1021/acsnano.9b03144

Resolving Single-Nanoconstruct Dynamics during Targeting and Nontargeting Live-Cell Membrane Interactions

Debanjan Bhowmik, Kayla S.B. Culver, Tingting Liu, Teri W. Odom^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

This paper describes how differences in the dynamics of targeting and nontargeting constructs can provide information on nanoparticle (NP)-cell interactions. We probed translational and rotational dynamics of functionalized Au nanostar (AuNS) nanoconstructs interacting with cells in serum-containing medium. We found that AuNS with targeting ligands had a larger dynamical footprint and faster rotational speed on cell membranes expressing human epidermal growth factor receptor 2 (HER-2) receptors compared to that of AuNS with nontargeting ligands. Targeting and nontargeting nanoconstructs displayed distinct membrane dynamics despite their similar protein adsorption profiles, which suggests that targeted interactions are preserved even in the presence of a protein corona. The high sensitivity of single-NP dynamics can be used to compare different nanoconstruct properties (such as NP size, shape, and surface chemistry) to improve their design as delivery vehicles.

Original language	English (US)
Pages (from-to)	13637-13644
Number of pages	8
Journal	ACS nano
Volume	13
Issue number	12
DOIs	https://doi.org/10.1021/acsnano.9b03144
State	Published - Dec 24 2019

Keywords

differential interference contrast microscopy
membrane-receptor interactions
protein corona
single-particle dynamics
targeting and nontargeting nanoconstructs

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)
Materials Science(all)

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10.1021/acsnano.9b03144

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title = "Resolving Single-Nanoconstruct Dynamics during Targeting and Nontargeting Live-Cell Membrane Interactions",

abstract = "This paper describes how differences in the dynamics of targeting and nontargeting constructs can provide information on nanoparticle (NP)-cell interactions. We probed translational and rotational dynamics of functionalized Au nanostar (AuNS) nanoconstructs interacting with cells in serum-containing medium. We found that AuNS with targeting ligands had a larger dynamical footprint and faster rotational speed on cell membranes expressing human epidermal growth factor receptor 2 (HER-2) receptors compared to that of AuNS with nontargeting ligands. Targeting and nontargeting nanoconstructs displayed distinct membrane dynamics despite their similar protein adsorption profiles, which suggests that targeted interactions are preserved even in the presence of a protein corona. The high sensitivity of single-NP dynamics can be used to compare different nanoconstruct properties (such as NP size, shape, and surface chemistry) to improve their design as delivery vehicles.",

keywords = "differential interference contrast microscopy, membrane-receptor interactions, protein corona, single-particle dynamics, targeting and nontargeting nanoconstructs",

author = "Debanjan Bhowmik and Culver, {Kayla S.B.} and Tingting Liu and Odom, {Teri W.}",

note = "Funding Information: This work was supported by NIH Grant R01GM115763 (D.B., K.S.B.C., T.L.). Nanoprobe analysis was performed at the Northwestern University Quantitative Bioelement Imaging Center generously supported by NASA Ames Research Center NNA06CB93G. The aptamer loading analysis was performed at the Northwestern University High Throughput Analysis Laboratory. This work made use of proteomics services performed by the Northwestern Proteomics Core Facility, generously supported by NCI CCSG P30 CA060553 awarded to the Robert H. Lurie Comprehensive Cancer Center and the National Resource for Translational and Developmental Proteomics supported by P41 GM108569. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

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N1 - Funding Information: This work was supported by NIH Grant R01GM115763 (D.B., K.S.B.C., T.L.). Nanoprobe analysis was performed at the Northwestern University Quantitative Bioelement Imaging Center generously supported by NASA Ames Research Center NNA06CB93G. The aptamer loading analysis was performed at the Northwestern University High Throughput Analysis Laboratory. This work made use of proteomics services performed by the Northwestern Proteomics Core Facility, generously supported by NCI CCSG P30 CA060553 awarded to the Robert H. Lurie Comprehensive Cancer Center and the National Resource for Translational and Developmental Proteomics supported by P41 GM108569. Publisher Copyright: © 2019 American Chemical Society.

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N2 - This paper describes how differences in the dynamics of targeting and nontargeting constructs can provide information on nanoparticle (NP)-cell interactions. We probed translational and rotational dynamics of functionalized Au nanostar (AuNS) nanoconstructs interacting with cells in serum-containing medium. We found that AuNS with targeting ligands had a larger dynamical footprint and faster rotational speed on cell membranes expressing human epidermal growth factor receptor 2 (HER-2) receptors compared to that of AuNS with nontargeting ligands. Targeting and nontargeting nanoconstructs displayed distinct membrane dynamics despite their similar protein adsorption profiles, which suggests that targeted interactions are preserved even in the presence of a protein corona. The high sensitivity of single-NP dynamics can be used to compare different nanoconstruct properties (such as NP size, shape, and surface chemistry) to improve their design as delivery vehicles.

AB - This paper describes how differences in the dynamics of targeting and nontargeting constructs can provide information on nanoparticle (NP)-cell interactions. We probed translational and rotational dynamics of functionalized Au nanostar (AuNS) nanoconstructs interacting with cells in serum-containing medium. We found that AuNS with targeting ligands had a larger dynamical footprint and faster rotational speed on cell membranes expressing human epidermal growth factor receptor 2 (HER-2) receptors compared to that of AuNS with nontargeting ligands. Targeting and nontargeting nanoconstructs displayed distinct membrane dynamics despite their similar protein adsorption profiles, which suggests that targeted interactions are preserved even in the presence of a protein corona. The high sensitivity of single-NP dynamics can be used to compare different nanoconstruct properties (such as NP size, shape, and surface chemistry) to improve their design as delivery vehicles.

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Resolving Single-Nanoconstruct Dynamics during Targeting and Nontargeting Live-Cell Membrane Interactions

Abstract

Keywords

ASJC Scopus subject areas

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