Respiratory colonization and short-term temporal changes in the urinary metabolome of children

Lilliam Ambroggio; Todd A. Florin; Kayla Williamson; Cory Pfefferman; Brandie D. Wagner; Larisa Yeomans; Jae Hyun Kim; Heidi Sucharew; Maurizio Macaluso; Richard M. Ruddy; Samir S. Shah; Kathleen A. Stringer

doi:10.3390/metabo11080500

Respiratory colonization and short-term temporal changes in the urinary metabolome of children

Lilliam Ambroggio^*, Todd A. Florin, Kayla Williamson, Cory Pfefferman, Brandie D. Wagner, Larisa Yeomans, Jae Hyun Kim, Heidi Sucharew, Maurizio Macaluso, Richard M. Ruddy, Samir S. Shah, Kathleen A. Stringer

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The human metabolome may vary based on age, over time, and in the presence of viral carriage and bacterial colonization—a common scenario in children. We used nuclear magnetic resonance spectroscopy to identify and quantify urinary metabolites of children without signs or symptoms of respiratory illness. A urine sample and two nasopharyngeal swabs were collected to test for respiratory viral pathogens and colonization by Streptococcus pneumoniae (Sp). Urine samples were collected at the initial visit, 24 h post-enrollment, and 10–14 days post-enrollment. Of the 122 children enrolled, 24% had a virus detected and 19.7% had Sp detected. Intraclass correlation coefficients demonstrated greater within-subject versus between-subject variability for all metabolites detected. In linear mixed models adjusted for age, time, history of asthma, Sp, and viruses, 1-methylnicotinamide was increased by 50% in children with Sp and decreased by 35% in children with rhinovirus/enterovirus. Children with Sp had 83% higher levels of trimethylamine-N-oxide compared with those without Sp. However, when adjusting for multiple comparisons, the association was no longer statistically significant. In conclusion, there appear to be short-term changes within the urinary metabolome of healthy children, but levels of metabolites did not statistically differ in children with viral carriage or Sp detected.

Original language	English (US)
Article number	500
Journal	Metabolites
Volume	11
Issue number	8
DOIs	https://doi.org/10.3390/metabo11080500
State	Published - Aug 2021

Funding

Conflicts of Interest: L.A. has received research grant support through Children’s Hospital Colorado from Pfizer Inc. for an unrelated study. Funding: L.A. received funding from the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), grant number K01 AI125413. T.A.F.’s efforts were supported by the NIAID, grant number K23 AI 121325. K.A.S.’s efforts were supported by a grant from the National Institute of General Medical Sciences (NIGMS), R01GM111400. The content is solely the responsibility of the authors, and does not necessarily represent the official views of the NIAID, NIGMS, or the NIH.

Keywords

Healthy
Metabolome
Nuclear magnetic resonance
Pediatric
Rhinovirus/enterovirus
Streptococcus pneumoniae

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/metabo11080500

Cite this

@article{2f094efc4baa4e86b2807bc21ca7df6a,

title = "Respiratory colonization and short-term temporal changes in the urinary metabolome of children",

abstract = "The human metabolome may vary based on age, over time, and in the presence of viral carriage and bacterial colonization—a common scenario in children. We used nuclear magnetic resonance spectroscopy to identify and quantify urinary metabolites of children without signs or symptoms of respiratory illness. A urine sample and two nasopharyngeal swabs were collected to test for respiratory viral pathogens and colonization by Streptococcus pneumoniae (Sp). Urine samples were collected at the initial visit, 24 h post-enrollment, and 10–14 days post-enrollment. Of the 122 children enrolled, 24% had a virus detected and 19.7% had Sp detected. Intraclass correlation coefficients demonstrated greater within-subject versus between-subject variability for all metabolites detected. In linear mixed models adjusted for age, time, history of asthma, Sp, and viruses, 1-methylnicotinamide was increased by 50% in children with Sp and decreased by 35% in children with rhinovirus/enterovirus. Children with Sp had 83% higher levels of trimethylamine-N-oxide compared with those without Sp. However, when adjusting for multiple comparisons, the association was no longer statistically significant. In conclusion, there appear to be short-term changes within the urinary metabolome of healthy children, but levels of metabolites did not statistically differ in children with viral carriage or Sp detected.",

keywords = "Healthy, Metabolome, Nuclear magnetic resonance, Pediatric, Rhinovirus/enterovirus, Streptococcus pneumoniae",

author = "Lilliam Ambroggio and Florin, {Todd A.} and Kayla Williamson and Cory Pfefferman and Wagner, {Brandie D.} and Larisa Yeomans and Kim, {Jae Hyun} and Heidi Sucharew and Maurizio Macaluso and Ruddy, {Richard M.} and Shah, {Samir S.} and Stringer, {Kathleen A.}",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = aug,

doi = "10.3390/metabo11080500",

language = "English (US)",

volume = "11",

journal = "Metabolites",

issn = "2218-1989",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "8",

}

TY - JOUR

T1 - Respiratory colonization and short-term temporal changes in the urinary metabolome of children

AU - Ambroggio, Lilliam

AU - Florin, Todd A.

AU - Williamson, Kayla

AU - Pfefferman, Cory

AU - Wagner, Brandie D.

AU - Yeomans, Larisa

AU - Kim, Jae Hyun

AU - Sucharew, Heidi

AU - Macaluso, Maurizio

AU - Ruddy, Richard M.

AU - Shah, Samir S.

AU - Stringer, Kathleen A.

PY - 2021/8

Y1 - 2021/8

N2 - The human metabolome may vary based on age, over time, and in the presence of viral carriage and bacterial colonization—a common scenario in children. We used nuclear magnetic resonance spectroscopy to identify and quantify urinary metabolites of children without signs or symptoms of respiratory illness. A urine sample and two nasopharyngeal swabs were collected to test for respiratory viral pathogens and colonization by Streptococcus pneumoniae (Sp). Urine samples were collected at the initial visit, 24 h post-enrollment, and 10–14 days post-enrollment. Of the 122 children enrolled, 24% had a virus detected and 19.7% had Sp detected. Intraclass correlation coefficients demonstrated greater within-subject versus between-subject variability for all metabolites detected. In linear mixed models adjusted for age, time, history of asthma, Sp, and viruses, 1-methylnicotinamide was increased by 50% in children with Sp and decreased by 35% in children with rhinovirus/enterovirus. Children with Sp had 83% higher levels of trimethylamine-N-oxide compared with those without Sp. However, when adjusting for multiple comparisons, the association was no longer statistically significant. In conclusion, there appear to be short-term changes within the urinary metabolome of healthy children, but levels of metabolites did not statistically differ in children with viral carriage or Sp detected.

AB - The human metabolome may vary based on age, over time, and in the presence of viral carriage and bacterial colonization—a common scenario in children. We used nuclear magnetic resonance spectroscopy to identify and quantify urinary metabolites of children without signs or symptoms of respiratory illness. A urine sample and two nasopharyngeal swabs were collected to test for respiratory viral pathogens and colonization by Streptococcus pneumoniae (Sp). Urine samples were collected at the initial visit, 24 h post-enrollment, and 10–14 days post-enrollment. Of the 122 children enrolled, 24% had a virus detected and 19.7% had Sp detected. Intraclass correlation coefficients demonstrated greater within-subject versus between-subject variability for all metabolites detected. In linear mixed models adjusted for age, time, history of asthma, Sp, and viruses, 1-methylnicotinamide was increased by 50% in children with Sp and decreased by 35% in children with rhinovirus/enterovirus. Children with Sp had 83% higher levels of trimethylamine-N-oxide compared with those without Sp. However, when adjusting for multiple comparisons, the association was no longer statistically significant. In conclusion, there appear to be short-term changes within the urinary metabolome of healthy children, but levels of metabolites did not statistically differ in children with viral carriage or Sp detected.

KW - Healthy

KW - Metabolome

KW - Nuclear magnetic resonance

KW - Pediatric

KW - Rhinovirus/enterovirus

KW - Streptococcus pneumoniae

UR - http://www.scopus.com/inward/record.url?scp=85111752433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85111752433&partnerID=8YFLogxK

U2 - 10.3390/metabo11080500

DO - 10.3390/metabo11080500

M3 - Article

C2 - 34436441

AN - SCOPUS:85111752433

SN - 2218-1989

VL - 11

JO - Metabolites

JF - Metabolites

IS - 8

M1 - 500

ER -

Respiratory colonization and short-term temporal changes in the urinary metabolome of children

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this