Response of anterior pituitary cells to culture media

M. J. Cronin; J. Weiss; A. D. Rogol; A. J. Baertschi

doi:10.1080/07435808709023665

Response of anterior pituitary cells to culture media

M. J. Cronin, J. Weiss, A. D. Rogol, A. J. Baertschi

Medicine, Endocrinology Division

Research output: Contribution to journal › Article › peer-review

Abstract

We examined the ability of four commonly used culture media to support prolactin (PRL), growth hormone (GH), and adrenocorticotropic hormone (ACTH) release, as well as the inhibitory PRL response to dopamine. After a week of primary culture, rat anterior pituitary cells from both genders were studied over a 4 hour period. Whereas ACTH secretion was similar across the various media, PRL and GH release were lessened with M199 and F10, respectively. Dopamine inhibited PRL release under all media conditions which was inconsistant with the reported lack of a dopamine effect with RPMI-1640 medium. These data confirm the postulate that media culture conditions can determine the degree of expression of constituitive phenotypes in anterior pituitary cells.

Original language	English (US)
Pages (from-to)	85-95
Number of pages	11
Journal	Endocrine Research
Volume	13
Issue number	1
DOIs	https://doi.org/10.1080/07435808709023665
State	Published - Jan 1987

Funding

ACKNOWLEDGPiENTS We gratefully acknowledge the excellent technical assistance of Gwen Baber, Richard Pence and Catherine Cassada. This work was supported by grants NS18409, RCDA lK04NS00601, HL31133, CITBI085025.

ASJC Scopus subject areas

Endocrinology

Access to Document

10.1080/07435808709023665

Cite this

@article{7b6645dab2c84adaabf33295d11c8097,

title = "Response of anterior pituitary cells to culture media",

abstract = "We examined the ability of four commonly used culture media to support prolactin (PRL), growth hormone (GH), and adrenocorticotropic hormone (ACTH) release, as well as the inhibitory PRL response to dopamine. After a week of primary culture, rat anterior pituitary cells from both genders were studied over a 4 hour period. Whereas ACTH secretion was similar across the various media, PRL and GH release were lessened with M199 and F10, respectively. Dopamine inhibited PRL release under all media conditions which was inconsistant with the reported lack of a dopamine effect with RPMI-1640 medium. These data confirm the postulate that media culture conditions can determine the degree of expression of constituitive phenotypes in anterior pituitary cells.",

author = "Cronin, {M. J.} and J. Weiss and Rogol, {A. D.} and Baertschi, {A. J.}",

note = "Funding Information: ACKNOWLEDGPiENTS We gratefully acknowledge the excellent technical assistance of Gwen Baber, Richard Pence and Catherine Cassada. This work was supported by grants NS18409, RCDA lK04NS00601, HL31133, CITBI085025.",

year = "1987",

month = jan,

doi = "10.1080/07435808709023665",

language = "English (US)",

volume = "13",

pages = "85--95",

journal = "Endocrine Research",

issn = "0743-5800",

publisher = "Taylor & Francis Group LLC Philadelphia",

number = "1",

}

TY - JOUR

T1 - Response of anterior pituitary cells to culture media

AU - Cronin, M. J.

AU - Weiss, J.

AU - Rogol, A. D.

AU - Baertschi, A. J.

N1 - Funding Information: ACKNOWLEDGPiENTS We gratefully acknowledge the excellent technical assistance of Gwen Baber, Richard Pence and Catherine Cassada. This work was supported by grants NS18409, RCDA lK04NS00601, HL31133, CITBI085025.

PY - 1987/1

Y1 - 1987/1

N2 - We examined the ability of four commonly used culture media to support prolactin (PRL), growth hormone (GH), and adrenocorticotropic hormone (ACTH) release, as well as the inhibitory PRL response to dopamine. After a week of primary culture, rat anterior pituitary cells from both genders were studied over a 4 hour period. Whereas ACTH secretion was similar across the various media, PRL and GH release were lessened with M199 and F10, respectively. Dopamine inhibited PRL release under all media conditions which was inconsistant with the reported lack of a dopamine effect with RPMI-1640 medium. These data confirm the postulate that media culture conditions can determine the degree of expression of constituitive phenotypes in anterior pituitary cells.

AB - We examined the ability of four commonly used culture media to support prolactin (PRL), growth hormone (GH), and adrenocorticotropic hormone (ACTH) release, as well as the inhibitory PRL response to dopamine. After a week of primary culture, rat anterior pituitary cells from both genders were studied over a 4 hour period. Whereas ACTH secretion was similar across the various media, PRL and GH release were lessened with M199 and F10, respectively. Dopamine inhibited PRL release under all media conditions which was inconsistant with the reported lack of a dopamine effect with RPMI-1640 medium. These data confirm the postulate that media culture conditions can determine the degree of expression of constituitive phenotypes in anterior pituitary cells.

UR - http://www.scopus.com/inward/record.url?scp=0023140482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023140482&partnerID=8YFLogxK

U2 - 10.1080/07435808709023665

DO - 10.1080/07435808709023665

M3 - Article

C2 - 3034559

AN - SCOPUS:0023140482

SN - 0743-5800

VL - 13

SP - 85

EP - 95

JO - Endocrine Research

JF - Endocrine Research

IS - 1

ER -

Response of anterior pituitary cells to culture media

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this