Response of anterior pituitary cells to culture media

M. J. Cronin, J. Weiss, A. D. Rogol, A. J. Baertschi

Research output: Contribution to journalArticlepeer-review

Abstract

We examined the ability of four commonly used culture media to support prolactin (PRL), growth hormone (GH), and adrenocorticotropic hormone (ACTH) release, as well as the inhibitory PRL response to dopamine. After a week of primary culture, rat anterior pituitary cells from both genders were studied over a 4 hour period. Whereas ACTH secretion was similar across the various media, PRL and GH release were lessened with M199 and F10, respectively. Dopamine inhibited PRL release under all media conditions which was inconsistant with the reported lack of a dopamine effect with RPMI-1640 medium. These data confirm the postulate that media culture conditions can determine the degree of expression of constituitive phenotypes in anterior pituitary cells.

Original languageEnglish (US)
Pages (from-to)85-95
Number of pages11
JournalEndocrine Research
Volume13
Issue number1
DOIs
StatePublished - Jan 1987

ASJC Scopus subject areas

  • Endocrinology

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