Responses of osteoblastic cells (UMR 106) exposed to elevated extracellular calcium

Craig S. Haga, Paula H. Stem

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Bone cells can be exposed to high calcium in the course of endodontic treatment. To investigate the effects of high calcium on bone cell function, responses of a rat osteoblast cell line (UMR 106) were examined. Responsiveness of the cells to parathyroid hormone, prostaglandin F, and ionomycin was assessed by measuring calcium transients elicited by these stimuli. Raising the medium calcium from 1.8 to 50 mm did not alter cell responsiveness. Pretreatment of the cells with the calcium pump inhibitor sodium vanadate prevented parathyroid hormone effects and slightly decreased prostaglandin F effects in both normal and high calcium. The effect of ionomycin was prolonged in high calcium when vanadate was present. The results suggest that cells of the osteoblast phenotype can maintain calcium signaling in the presence of high extracellular calcium. These processes could play a role in the therapeutic effectiveness of high calcium in endodontic treatment.

Original languageEnglish (US)
Pages (from-to)462-465
Number of pages4
JournalJournal of endodontics
Issue number9
StatePublished - Sep 1993

ASJC Scopus subject areas

  • General Dentistry


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