TY - JOUR
T1 - Resveratrol increases glutamate uptake and glutamine synthetase activity in C6 glioma cells
AU - dos Santos, André Quincozes
AU - Nardin, Patrícia
AU - Funchal, Cláudia
AU - Vieira de Almeida, Lucia Maria
AU - Jacques-Silva, Maria Caroline
AU - Wofchuk, Susana T.
AU - Gonçalves, Carlos Alberto
AU - Gottfried, Carmem
N1 - Funding Information:
This work was supported by the brazilian funding agents; Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Pró-Reitoria de Pesquisa da Universidade Federal do Rio Grande do Sul (PROPESQ-UFRGS).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/9/15
Y1 - 2006/9/15
N2 - Resveratrol, a phytoalexin found mainly in grapes, is a promising natural product with anti-cancer and cardio-protective activities. Here, we investigated, in C6 glioma cells, the effect of resveratrol on some specific parameters of astrocyte activity (glutamate uptake, glutamine synthetase and secretion of S100B, a neurotrophic cytokine) commonly associated with the protective role of these cells. Cell proliferation was significantly decreased by 8% and 26%, following 24 h of treatment with 100 and 250 μM resveratrol. Extracellular S100B increased after 48 h of resveratrol exposure. Short-term resveratrol exposure (from 1 to 100 μM) induced a linear increase in glutamate uptake (up to 50% at 100 μM resveratrol) and in glutamine synthetase activity. Changes in these glial activities can contribute to the protective role of astrocytes in brain injury conditions, reinforcing the putative use of this compound in the therapeutic arsenal against neurodegenerative diseases and ischemic disorders.
AB - Resveratrol, a phytoalexin found mainly in grapes, is a promising natural product with anti-cancer and cardio-protective activities. Here, we investigated, in C6 glioma cells, the effect of resveratrol on some specific parameters of astrocyte activity (glutamate uptake, glutamine synthetase and secretion of S100B, a neurotrophic cytokine) commonly associated with the protective role of these cells. Cell proliferation was significantly decreased by 8% and 26%, following 24 h of treatment with 100 and 250 μM resveratrol. Extracellular S100B increased after 48 h of resveratrol exposure. Short-term resveratrol exposure (from 1 to 100 μM) induced a linear increase in glutamate uptake (up to 50% at 100 μM resveratrol) and in glutamine synthetase activity. Changes in these glial activities can contribute to the protective role of astrocytes in brain injury conditions, reinforcing the putative use of this compound in the therapeutic arsenal against neurodegenerative diseases and ischemic disorders.
KW - C6 glioma
KW - GFAP
KW - Glutamate uptake
KW - Glutamine synthetase
KW - Resveratrol
KW - S100B
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U2 - 10.1016/j.abb.2006.06.025
DO - 10.1016/j.abb.2006.06.025
M3 - Article
C2 - 16904623
AN - SCOPUS:33748422199
SN - 0003-9861
VL - 453
SP - 161
EP - 167
JO - Archives of biochemistry and biophysics
JF - Archives of biochemistry and biophysics
IS - 2
ER -
