Resveratrol increases glutamate uptake and glutamine synthetase activity in C6 glioma cells

André Quincozes dos Santos, Patrícia Nardin, Cláudia Funchal, Lucia Maria Vieira de Almeida, Maria Caroline Jacques-Silva, Susana T. Wofchuk, Carlos Alberto Gonçalves, Carmem Gottfried*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

97 Scopus citations


Resveratrol, a phytoalexin found mainly in grapes, is a promising natural product with anti-cancer and cardio-protective activities. Here, we investigated, in C6 glioma cells, the effect of resveratrol on some specific parameters of astrocyte activity (glutamate uptake, glutamine synthetase and secretion of S100B, a neurotrophic cytokine) commonly associated with the protective role of these cells. Cell proliferation was significantly decreased by 8% and 26%, following 24 h of treatment with 100 and 250 μM resveratrol. Extracellular S100B increased after 48 h of resveratrol exposure. Short-term resveratrol exposure (from 1 to 100 μM) induced a linear increase in glutamate uptake (up to 50% at 100 μM resveratrol) and in glutamine synthetase activity. Changes in these glial activities can contribute to the protective role of astrocytes in brain injury conditions, reinforcing the putative use of this compound in the therapeutic arsenal against neurodegenerative diseases and ischemic disorders.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalArchives of biochemistry and biophysics
Issue number2
StatePublished - Sep 15 2006


  • C6 glioma
  • GFAP
  • Glutamate uptake
  • Glutamine synthetase
  • Resveratrol
  • S100B

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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