Resveratrol increases glutamate uptake, glutathione content, and S100B secretion in cortical astrocyte cultures

Lúcia Maria Vieira De Almeida, Cristopher Celintano Piñeiro, Marina Concli Leite, Giovana Brolese, Francine Tramontina, Ana Maria Feoli, Carmem Gottfried, Carlos Alberto Gonçalves*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


Resveratrol (3,5,4′-trihydroxy-trans-stilbene) is a polyphenol present in grapes and red wine, which has antioxidant properties and a wide range of other biological effects. In this study, we investigated the effect of resveratrol, in a concentration range of 10-250 μM, on primary cortical astrocytes; evaluating cell morphology, parameters of glutamate metabolism such as glutamate uptake, glutamine synthetase activity and glutathione total content, and S100B secretion. Astrocyte cultures were prepared of cerebral cortex from neonate Wistar rats. Morphology was evaluated by phase-contrast microscopy and immunocytochemistry for glial fibrillary acidic protein (GFAP). Glutamate uptake was measured using l-[2,3-3H]glutamate. Glutamine synthetase and content of glutathione were measured by enzymatic colorimetric assays. S100B content was determined by ELISA. Typical polygonal morphology becomes stellated when astrocyte cultures were exposed to 250 μM resveratrol for 24 h. At concentration of 25 μM, resveratrol was able to increase glutamate uptake and glutathione content. Conversely, at 250 μM, resveratrol decreased glutamate uptake. Unexpectedly, resveratrol at this high concentration increased glutamine synthetase activity. Extracellular S100B increased from 50 μM upwards. Our findings reinforce the protective role of this compound in some brain disorders, particularly those involving glutamate toxicity. However, the underlying mechanisms of these changes are not clear at the moment and it is necessary caution with its administration because elevated levels of this compound could contribute to aggravate these conditions.

Original languageEnglish (US)
Pages (from-to)661-668
Number of pages8
JournalCellular and molecular neurobiology
Issue number5
StatePublished - Jul 2007


  • Astrocyte
  • GFAP
  • Glutamate uptake
  • Glutathione
  • Resveratrol
  • S100B

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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