Revealing nanoscale mineralization pathways of hydroxyapatite using in situ liquid cell transmission electron microscopy

Kun He, Michal Sawczyk, Cong Liu, Y. Yuan, Boao Song, Ram Deivanayagam, Anmin Nie, Xiaobing Hu, Vinayak P. Dravid, Jun Lu, Cortino Sukotjo, Yu Peng Lu, Petr Krá, Tolou Shokuhfar*, Reza Shahbazian-Yassar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

To treat impairments in hard tissues or overcome pathological calcification in soft tissues, a detailed understanding of mineralization pathways of calcium phosphate materials is needed. Here, we report a detailed mechanistic study of hydroxyapatite (HA) mineralization pathways in an artificial saliva solution via in situ liquid cell transmission electron microscopy (TEM). It is found that the mineralization of HA starts by forming ion-rich and ion-poor solutions in the saliva solution, followed by coexistence of the classical and nonclassical nucleation processes. For the nonclassical path, amorphous calcium phosphate (ACP) functions as the substrate for HA nucleation on the ACP surface, while the classical path features direct HA nucleation from the solution. The growth of HA crystals on the surface of ACP is accompanied by the ACP dissolution process. The discoveries reported in this work are important to understand the physiological and pathological formation of HA minerals, as well as to engineer the biomineralization process for bone healing and hard tissue repairs.

Original languageEnglish (US)
Article numberaaz7524
JournalScience Advances
Volume6
Issue number47
DOIs
StatePublished - Nov 18 2020

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Revealing nanoscale mineralization pathways of hydroxyapatite using in situ liquid cell transmission electron microscopy'. Together they form a unique fingerprint.

Cite this