Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase

William T. Tse, Bernard G. Forget*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

We report the ability of Taq polymerase to directly transcribe RNA templates in vitro. We have made use of this finding to develop a single-step protocol for amplification of RNA transcripts. The method was shown to require only subnanogram amounts of total cellular RNA as starting material. A microassay was developed in which RNA can be extracted from one drop of blood or 1000 cultured cells, and analyzed for the expression of a specific gene.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalGene
Volume88
Issue number2
DOIs
StatePublished - Apr 16 1990

Keywords

  • Recombinant DNA
  • gene expression
  • human erythroleukemia cells
  • messenger RNA
  • microassay
  • polymerase chain reaction
  • β-spectrin-encoding gene

ASJC Scopus subject areas

  • Genetics

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