Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase

William T. Tse; Bernard G. Forget

doi:10.1016/0378-1119(90)90047-U

Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase

William T. Tse, Bernard G. Forget^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

We report the ability of Taq polymerase to directly transcribe RNA templates in vitro. We have made use of this finding to develop a single-step protocol for amplification of RNA transcripts. The method was shown to require only subnanogram amounts of total cellular RNA as starting material. A microassay was developed in which RNA can be extracted from one drop of blood or 1000 cultured cells, and analyzed for the expression of a specific gene.

Original language	English (US)
Pages (from-to)	293-296
Number of pages	4
Journal	Gene
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(90)90047-U
State	Published - Apr 16 1990

Keywords

Recombinant DNA
gene expression
human erythroleukemia cells
messenger RNA
microassay
polymerase chain reaction
β-spectrin-encoding gene

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(90)90047-U

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abstract = "We report the ability of Taq polymerase to directly transcribe RNA templates in vitro. We have made use of this finding to develop a single-step protocol for amplification of RNA transcripts. The method was shown to require only subnanogram amounts of total cellular RNA as starting material. A microassay was developed in which RNA can be extracted from one drop of blood or 1000 cultured cells, and analyzed for the expression of a specific gene.",

keywords = "Recombinant DNA, gene expression, human erythroleukemia cells, messenger RNA, microassay, polymerase chain reaction, β-spectrin-encoding gene",

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T1 - Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase

AU - Tse, William T.

AU - Forget, Bernard G.

PY - 1990/4/16

Y1 - 1990/4/16

N2 - We report the ability of Taq polymerase to directly transcribe RNA templates in vitro. We have made use of this finding to develop a single-step protocol for amplification of RNA transcripts. The method was shown to require only subnanogram amounts of total cellular RNA as starting material. A microassay was developed in which RNA can be extracted from one drop of blood or 1000 cultured cells, and analyzed for the expression of a specific gene.

AB - We report the ability of Taq polymerase to directly transcribe RNA templates in vitro. We have made use of this finding to develop a single-step protocol for amplification of RNA transcripts. The method was shown to require only subnanogram amounts of total cellular RNA as starting material. A microassay was developed in which RNA can be extracted from one drop of blood or 1000 cultured cells, and analyzed for the expression of a specific gene.

KW - Recombinant DNA

KW - gene expression

KW - human erythroleukemia cells

KW - messenger RNA

KW - microassay

KW - polymerase chain reaction

KW - β-spectrin-encoding gene

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JO - Gene

JF - Gene

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