Reversible and irreversible unfolding of eukaryote chromosomes by force

John F. Marko; Michael Poirier; Sertac Eroglu; Didier Chatenay

Reversible and irreversible unfolding of eukaryote chromosomes by force

John F. Marko^*, Michael Poirier, Sertac Eroglu, Didier Chatenay

^*Corresponding author for this work

Molecular Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have carried out studies of metaphase chromosomes in primary explant cultures of newt lung epithelia, using in situ microsurgery and force measurement. The chromosomes are elastic over a five-fold range of extension, with a force constant of about one nanonewton (nN). For larger extensions, chromosomes are permanently elongated; if stretched beyond 30 times, their cross-sectional diameter is increased after relaxation. Fluorescence studies indicate that histone proteins are not being removed. We conclude that we are first unfolding and then breaking chromosome-folding proteins by extension.

Original language	English (US)
Title of host publication	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Publisher	IEEE
Number of pages	1
ISBN (Print)	0780356756
State	Published - Dec 1 1999
Event	Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA Duration: Oct 13 1999 → Oct 16 1999

Publication series

Name	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume	1
ISSN (Print)	0589-1019

Other

Other	Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
City	Atlanta, GA, USA
Period	10/13/99 → 10/16/99

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

Cite this

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title = "Reversible and irreversible unfolding of eukaryote chromosomes by force",

abstract = "We have carried out studies of metaphase chromosomes in primary explant cultures of newt lung epithelia, using in situ microsurgery and force measurement. The chromosomes are elastic over a five-fold range of extension, with a force constant of about one nanonewton (nN). For larger extensions, chromosomes are permanently elongated; if stretched beyond 30 times, their cross-sectional diameter is increased after relaxation. Fluorescence studies indicate that histone proteins are not being removed. We conclude that we are first unfolding and then breaking chromosome-folding proteins by extension.",

author = "Marko, {John F.} and Michael Poirier and Sertac Eroglu and Didier Chatenay",

year = "1999",

month = dec,

day = "1",

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series = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

publisher = "IEEE",

booktitle = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

note = "Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) ; Conference date: 13-10-1999 Through 16-10-1999",

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Marko, JF, Poirier, M, Eroglu, S & Chatenay, D 1999, Reversible and irreversible unfolding of eukaryote chromosomes by force. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 1, IEEE, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.

Reversible and irreversible unfolding of eukaryote chromosomes by force. / Marko, John F.; Poirier, Michael; Eroglu, Sertac et al.
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE, 1999. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Reversible and irreversible unfolding of eukaryote chromosomes by force

AU - Marko, John F.

AU - Poirier, Michael

AU - Eroglu, Sertac

AU - Chatenay, Didier

PY - 1999/12/1

Y1 - 1999/12/1

N2 - We have carried out studies of metaphase chromosomes in primary explant cultures of newt lung epithelia, using in situ microsurgery and force measurement. The chromosomes are elastic over a five-fold range of extension, with a force constant of about one nanonewton (nN). For larger extensions, chromosomes are permanently elongated; if stretched beyond 30 times, their cross-sectional diameter is increased after relaxation. Fluorescence studies indicate that histone proteins are not being removed. We conclude that we are first unfolding and then breaking chromosome-folding proteins by extension.

AB - We have carried out studies of metaphase chromosomes in primary explant cultures of newt lung epithelia, using in situ microsurgery and force measurement. The chromosomes are elastic over a five-fold range of extension, with a force constant of about one nanonewton (nN). For larger extensions, chromosomes are permanently elongated; if stretched beyond 30 times, their cross-sectional diameter is increased after relaxation. Fluorescence studies indicate that histone proteins are not being removed. We conclude that we are first unfolding and then breaking chromosome-folding proteins by extension.

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M3 - Conference contribution

AN - SCOPUS:0033335272

SN - 0780356756

T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

PB - IEEE

T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)

Y2 - 13 October 1999 through 16 October 1999

ER -

Reversible and irreversible unfolding of eukaryote chromosomes by force

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