Reversible and irreversible unfolding of eukaryote chromosomes by force

John F. Marko*, Michael Poirier, Sertac Eroglu, Didier Chatenay

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have carried out studies of metaphase chromosomes in primary explant cultures of newt lung epithelia, using in situ microsurgery and force measurement. The chromosomes are elastic over a five-fold range of extension, with a force constant of about one nanonewton (nN). For larger extensions, chromosomes are permanently elongated; if stretched beyond 30 times, their cross-sectional diameter is increased after relaxation. Fluorescence studies indicate that histone proteins are not being removed. We conclude that we are first unfolding and then breaking chromosome-folding proteins by extension.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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