Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams

P. Saggau*, N. Ball, P. Bianchini, J. Brockill, W. E. Brownell, J. R. Cotton, A. Diaspro, M. Duocastella, O. Gliko, V. Iyer, N. Orlova, G. D. Reddy, A. S. Tolias, D. Tsyboulski

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


This review presents a selection of advanced microscope designs employing acousto-optical deflectors (AODs). In the designs covered, AODs are used as tunable diffraction gratings to control complex illumination patterns at the Fourier plane of an objective lens. This approach allows us to generate desired illumination patterns at the focal plane of a light microscope. In simple terms, I will describe two established designs, the 3D Random-Access Multi-Photon Microscope and the Standing-Wave Super-Resolution Microscope, as well as two new schemes including the Random-Access STED Microscope and the Frequency-Encoded Multi-Beam Microscope. All instruments mentioned here were designed to overcome the throughput limitations of previously used light microscopes in experimental Neuroscience.

Original languageEnglish (US)
Title of host publicationFourteenth School on Acousto-Optics and Applications
EditorsIreneusz Grulkowski, Bogumil B. J. Linde, Marti Duocastella
ISBN (Electronic)9781510631830
StatePublished - 2019
Event14th School on Acousto-Optics and Applications, SAOA 2019 - Torun, Poland
Duration: Jun 24 2019Jun 27 2019

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


Conference14th School on Acousto-Optics and Applications, SAOA 2019


  • 3D scanning
  • Back focal plane pattern
  • Bidirectional light propagation
  • Chirped acoustic waves
  • Frequency-shifted multi-beam
  • Optical standing-wave pattern
  • Phase-Aligned averaging.
  • Stimulated emission depletion

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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