Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams

P. Saggau; N. Ball; P. Bianchini; J. Brockill; W. E. Brownell; J. R. Cotton; A. Diaspro; M. Duocastella; O. Gliko; V. Iyer; N. Orlova; G. D. Reddy; A. S. Tolias; D. Tsyboulski

doi:10.1117/12.2538419

Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams

P. Saggau^*, N. Ball, P. Bianchini, J. Brockill, W. E. Brownell, J. R. Cotton, A. Diaspro, M. Duocastella, O. Gliko, V. Iyer, N. Orlova, G. D. Reddy, A. S. Tolias, D. Tsyboulski

^*Corresponding author for this work

Physical Medicine and Rehabilitation

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

This review presents a selection of advanced microscope designs employing acousto-optical deflectors (AODs). In the designs covered, AODs are used as tunable diffraction gratings to control complex illumination patterns at the Fourier plane of an objective lens. This approach allows us to generate desired illumination patterns at the focal plane of a light microscope. In simple terms, I will describe two established designs, the 3D Random-Access Multi-Photon Microscope and the Standing-Wave Super-Resolution Microscope, as well as two new schemes including the Random-Access STED Microscope and the Frequency-Encoded Multi-Beam Microscope. All instruments mentioned here were designed to overcome the throughput limitations of previously used light microscopes in experimental Neuroscience.

Original language	English (US)
Title of host publication	Fourteenth School on Acousto-Optics and Applications
Editors	Ireneusz Grulkowski, Bogumil B. J. Linde, Marti Duocastella
Publisher	SPIE
ISBN (Electronic)	9781510631830
DOIs	https://doi.org/10.1117/12.2538419
State	Published - 2019
Event	14th School on Acousto-Optics and Applications, SAOA 2019 - Torun, Poland Duration: Jun 24 2019 → Jun 27 2019

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	11210
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	14th School on Acousto-Optics and Applications, SAOA 2019
Country/Territory	Poland
City	Torun
Period	6/24/19 → 6/27/19

Funding

This work was supported by the US National Science Foundation, Institute for Brain Science, and the Italian Institute of Technology. Dr. Saggau is supported by the Visiting Lecturer Program of SPIE.

Keywords

3D scanning
Back focal plane pattern
Bidirectional light propagation
Chirped acoustic waves
Frequency-shifted multi-beam
Optical standing-wave pattern
Phase-Aligned averaging.
Stimulated emission depletion

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.2538419

Cite this

Saggau, P., Ball, N., Bianchini, P., Brockill, J., Brownell, W. E., Cotton, J. R., Diaspro, A., Duocastella, M., Gliko, O., Iyer, V., Orlova, N., Reddy, G. D., Tolias, A. S., & Tsyboulski, D. (2019). Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams. In I. Grulkowski, B. B. J. Linde, & M. Duocastella (Eds.), Fourteenth School on Acousto-Optics and Applications Article 112100P (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11210). SPIE. https://doi.org/10.1117/12.2538419

Saggau, P. ; Ball, N. ; Bianchini, P. et al. / Review of acousto-optical devices in advanced microscopy : From 3D scanning via super-resolution to encoded multi-beams. Fourteenth School on Acousto-Optics and Applications. editor / Ireneusz Grulkowski ; Bogumil B. J. Linde ; Marti Duocastella. SPIE, 2019. (Proceedings of SPIE - The International Society for Optical Engineering).

@inproceedings{f48ab93c70974dca8537e01af97d5968,

title = "Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams",

abstract = "This review presents a selection of advanced microscope designs employing acousto-optical deflectors (AODs). In the designs covered, AODs are used as tunable diffraction gratings to control complex illumination patterns at the Fourier plane of an objective lens. This approach allows us to generate desired illumination patterns at the focal plane of a light microscope. In simple terms, I will describe two established designs, the 3D Random-Access Multi-Photon Microscope and the Standing-Wave Super-Resolution Microscope, as well as two new schemes including the Random-Access STED Microscope and the Frequency-Encoded Multi-Beam Microscope. All instruments mentioned here were designed to overcome the throughput limitations of previously used light microscopes in experimental Neuroscience.",

keywords = "3D scanning, Back focal plane pattern, Bidirectional light propagation, Chirped acoustic waves, Frequency-shifted multi-beam, Optical standing-wave pattern, Phase-Aligned averaging., Stimulated emission depletion",

author = "P. Saggau and N. Ball and P. Bianchini and J. Brockill and Brownell, {W. E.} and Cotton, {J. R.} and A. Diaspro and M. Duocastella and O. Gliko and V. Iyer and N. Orlova and Reddy, {G. D.} and Tolias, {A. S.} and D. Tsyboulski",

note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; 14th School on Acousto-Optics and Applications, SAOA 2019 ; Conference date: 24-06-2019 Through 27-06-2019",

year = "2019",

doi = "10.1117/12.2538419",

language = "English (US)",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

editor = "Ireneusz Grulkowski and Linde, {Bogumil B. J.} and Marti Duocastella",

booktitle = "Fourteenth School on Acousto-Optics and Applications",

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Saggau, P, Ball, N, Bianchini, P, Brockill, J, Brownell, WE, Cotton, JR, Diaspro, A, Duocastella, M, Gliko, O, Iyer, V, Orlova, N, Reddy, GD, Tolias, AS & Tsyboulski, D 2019, Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams. in I Grulkowski, BBJ Linde & M Duocastella (eds), Fourteenth School on Acousto-Optics and Applications., 112100P, Proceedings of SPIE - The International Society for Optical Engineering, vol. 11210, SPIE, 14th School on Acousto-Optics and Applications, SAOA 2019, Torun, Poland, 6/24/19. https://doi.org/10.1117/12.2538419

Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams. / Saggau, P.; Ball, N.; Bianchini, P. et al.
Fourteenth School on Acousto-Optics and Applications. ed. / Ireneusz Grulkowski; Bogumil B. J. Linde; Marti Duocastella. SPIE, 2019. 112100P (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11210).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Ball, N.

AU - Bianchini, P.

AU - Brockill, J.

AU - Brownell, W. E.

AU - Cotton, J. R.

AU - Diaspro, A.

AU - Duocastella, M.

AU - Gliko, O.

AU - Iyer, V.

AU - Orlova, N.

AU - Reddy, G. D.

AU - Tolias, A. S.

AU - Tsyboulski, D.

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AB - This review presents a selection of advanced microscope designs employing acousto-optical deflectors (AODs). In the designs covered, AODs are used as tunable diffraction gratings to control complex illumination patterns at the Fourier plane of an objective lens. This approach allows us to generate desired illumination patterns at the focal plane of a light microscope. In simple terms, I will describe two established designs, the 3D Random-Access Multi-Photon Microscope and the Standing-Wave Super-Resolution Microscope, as well as two new schemes including the Random-Access STED Microscope and the Frequency-Encoded Multi-Beam Microscope. All instruments mentioned here were designed to overcome the throughput limitations of previously used light microscopes in experimental Neuroscience.

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A2 - Linde, Bogumil B. J.

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Saggau P, Ball N, Bianchini P, Brockill J, Brownell WE, Cotton JR et al. Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams. In Grulkowski I, Linde BBJ, Duocastella M, editors, Fourteenth School on Acousto-Optics and Applications. SPIE. 2019. 112100P. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2538419

Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams

Abstract

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Keywords

ASJC Scopus subject areas

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