@inproceedings{276a997d89b54a38bc3de7058b48ee50,
title = "Reviving natural product and drug discovery pipelines with fungal artificial chromosome (FAC) technology",
abstract = "We have developed the fungal artificial chromosome [FAC, or autonomously replicating E. coli-fungal shuttle bacterial artificial chromosome (BAC)] technology. The FAC system or technology enables unbiased capturing of 263 full-length large secondary metabolite (SM) gene clusters (each lOOkb or larger) as individual FAC clones, from 6 sequenced fungal genomes for one-step transformation and heterologous expression. The FAC technology also enables precise SM gene and gene cluster editing and activating silent and cryptic SM gene clusters. We have demonstrated that the FAC technology is extremely effective for natural product and drug discovery by using a liquid chromatography-fourier transform mass spectrometer (LC-FTMS) with novel data analysis pipeline. This novel FAC platform provides a major advance in the study of fungal biosynthesis, enabling rapid detection of novel SMs, identification of their biosynthetic machinery, and dissection of their biosynthesis. As an example, three SM compounds: benzomalvin A, ophiobolin G, and a novel lipopeptide has been discovered from a FAC clone: AtFAC9J20 containing a super-SM gene cluster. This disruptive technology could revive natural product and drug discovery platform from sequenced fungi, fungal metagenomes and environmental microbial metagenomes.",
keywords = "Drug discovery, Fungal artificial chromosome (FAC), Natural product, Secondary metabolite (SM) gene clusters",
author = "Kenneth Clevenger and Bok, {Jin Woo} and Rosa Ye and Galen Miley and Thomas Velk and Cynthia Chen and Yang, {Ka Houa} and Peng Gao and Matthew Lamprecht and Paul Thomas and Islam, {Md Nurul} and Nancy Keller and Neil Kelleher and Chengcang Wu",
year = "2016",
language = "English (US)",
series = "Advanced Materials - TechConnect Briefs 2016",
publisher = "TechConnect",
pages = "226--227",
editor = "Matthew Laudon and Fiona Case and Fiona Case and Bart Romanowicz and Bart Romanowicz",
booktitle = "Biotech, Biomaterials and Biomedical - TechConnect Briefs 2016",
note = "10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 19th Annual Nanotech Conference and Expo, and the 2016 National SBIR/STTR Conference ; Conference date: 22-05-2016 Through 25-05-2016",
}