Ribosomal incorporation of cyclic β-amino acids into peptides using: In vitro translation

Joongoo Lee; Rafael Torres; Do Soon Kim; Michelle Byrom; Andrew D. Ellington; Michael C. Jewett

doi:10.1039/d0cc02121k

Ribosomal incorporation of cyclic β-amino acids into peptides using: In vitro translation

Joongoo Lee, Rafael Torres, Do Soon Kim, Michelle Byrom, Andrew D. Ellington, Michael C. Jewett^*

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We demonstrate in vitro incorporation of cyclic β-amino acids into peptides by the ribosome through genetic code reprogramming. Further, we show that incorporation efficiency can be increased through the addition of elongation factor P.

Original language	English (US)
Pages (from-to)	5597-5600
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	42
DOIs	https://doi.org/10.1039/d0cc02121k
State	Published - May 25 2020

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d0cc02121k

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title = "Ribosomal incorporation of cyclic β-amino acids into peptides using: In vitro translation",

abstract = "We demonstrate in vitro incorporation of cyclic β-amino acids into peptides by the ribosome through genetic code reprogramming. Further, we show that incorporation efficiency can be increased through the addition of elongation factor P.",

author = "Joongoo Lee and Rafael Torres and Kim, {Do Soon} and Michelle Byrom and Ellington, {Andrew D.} and Jewett, {Michael C.}",

note = "Funding Information: This work was supported by the Army Research Office Grant W911NF-16-1-0372 and the National Science Foundation Grant MCB-1716766. MCJ also thanks the David and Lucile Packard Foundation and the Camille Dreyfus Teacher-Scholar Program. This work made use of IMSERC at Northwestern. Publisher Copyright: This journal is {\textcopyright} The Royal Society of Chemistry.",

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T1 - Ribosomal incorporation of cyclic β-amino acids into peptides using

T2 - In vitro translation

AU - Lee, Joongoo

AU - Torres, Rafael

AU - Kim, Do Soon

AU - Byrom, Michelle

AU - Ellington, Andrew D.

AU - Jewett, Michael C.

N1 - Funding Information: This work was supported by the Army Research Office Grant W911NF-16-1-0372 and the National Science Foundation Grant MCB-1716766. MCJ also thanks the David and Lucile Packard Foundation and the Camille Dreyfus Teacher-Scholar Program. This work made use of IMSERC at Northwestern. Publisher Copyright: This journal is © The Royal Society of Chemistry.

PY - 2020/5/25

Y1 - 2020/5/25

N2 - We demonstrate in vitro incorporation of cyclic β-amino acids into peptides by the ribosome through genetic code reprogramming. Further, we show that incorporation efficiency can be increased through the addition of elongation factor P.

AB - We demonstrate in vitro incorporation of cyclic β-amino acids into peptides by the ribosome through genetic code reprogramming. Further, we show that incorporation efficiency can be increased through the addition of elongation factor P.

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JO - Chemical Communications

JF - Chemical Communications

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ER -

Ribosomal incorporation of cyclic β-amino acids into peptides using: In vitro translation

Abstract

ASJC Scopus subject areas

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