Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

Joongoo Lee, Kevin J. Schwarz, Hao Yu, Antje Krüger, Eric V. Anslyn, Andrew D. Ellington, Jeffrey S. Moore*, Michael C. Jewett

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

Original languageEnglish (US)
Pages (from-to)2661-2664
Number of pages4
JournalChemical Communications
Issue number21
StatePublished - Mar 14 2021

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry


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