Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

Joongoo Lee; Kevin J. Schwarz; Hao Yu; Antje Krüger; Eric V. Anslyn; Andrew D. Ellington; Jeffrey S. Moore; Michael C. Jewett

doi:10.1039/d0cc07740b

Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

Joongoo Lee, Kevin J. Schwarz, Hao Yu, Antje Krüger, Eric V. Anslyn, Andrew D. Ellington, Jeffrey S. Moore^*, Michael C. Jewett

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

Original language	English (US)
Pages (from-to)	2661-2664
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	21
DOIs	https://doi.org/10.1039/d0cc07740b
State	Published - Mar 14 2021

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/d0cc07740b

Cite this

@article{6f1cf57037a645529b5fd569256446f4,

title = "Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro",

abstract = "We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.",

author = "Joongoo Lee and Schwarz, {Kevin J.} and Hao Yu and Antje Kr{\"u}ger and Anslyn, {Eric V.} and Ellington, {Andrew D.} and Moore, {Jeffrey S.} and Jewett, {Michael C.}",

note = "Funding Information: JL, KJS, HY, AK, EVA, ADE, JSM, and MCJ conceived and designed the research. JL, KJS, HY, and AK performed experiments. All authors wrote and edited the manuscript. JS M and MCJ supervised this study. This work was supported by the Army Research Office Grant W911NF-16-1-0372. This work made use of IMSERC at Northwestern University. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = mar,

day = "14",

doi = "10.1039/d0cc07740b",

language = "English (US)",

volume = "57",

pages = "2661--2664",

journal = "Chemical Communications",

issn = "1359-7345",

number = "21",

}

TY - JOUR

T1 - Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

AU - Lee, Joongoo

AU - Schwarz, Kevin J.

AU - Yu, Hao

AU - Krüger, Antje

AU - Anslyn, Eric V.

AU - Ellington, Andrew D.

AU - Moore, Jeffrey S.

AU - Jewett, Michael C.

N1 - Funding Information: JL, KJS, HY, AK, EVA, ADE, JSM, and MCJ conceived and designed the research. JL, KJS, HY, and AK performed experiments. All authors wrote and edited the manuscript. JS M and MCJ supervised this study. This work was supported by the Army Research Office Grant W911NF-16-1-0372. This work made use of IMSERC at Northwestern University. Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2021/3/14

Y1 - 2021/3/14

N2 - We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

AB - We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosomein vitrothrough genetic code reprogramming.

UR - http://www.scopus.com/inward/record.url?scp=85102351901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85102351901&partnerID=8YFLogxK

U2 - 10.1039/d0cc07740b

DO - 10.1039/d0cc07740b

M3 - Article

C2 - 33592078

AN - SCOPUS:85102351901

SN - 1359-7345

VL - 57

SP - 2661

EP - 2664

JO - Chemical Communications

JF - Chemical Communications

IS - 21

ER -

Ribosome-mediated incorporation of fluorescent amino acids into peptidesin vitro

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this